This invention discloses a
cell proliferation culture device, belonging to the field of biomedical experimental equipment technology, comprising: a culture body, a microporous membrane, a
microfluidic channel, a
temperature control module, a sensor module, a sampler, and a
control unit. The
cell proliferation culture device of this invention can dynamically monitor the glucose consumption rate and lactate
production rate during
cell proliferation in real time, supports
pollution-free dynamic sampling and rapid microenvironment switching, and is particularly suitable for studying the interaction between SIRT2 and HKII to regulate aerobic
glycolysis and growth proliferation of
colorectal cancer cells. It also has advantages such as real-time monitoring, high sensitivity,
pollution-free sampling, and dynamic microenvironment control.