Serum-free medium for stem cells

Abstract

The invention discloses a serum-free medium for stem cells. The serum-free medium comprises a basic medium, an additive and a cytokine combination, wherein in terms of the volume of the basic medium as reference, the additive comprises transferrin in a volume ratio of 5%-10%, insulin with concentration of 5-15 mg / L, 2-4 mM of L-glutamine, growth factors with concentration of 5-15 ng / mL, BMP-5 antibody with concentration of 0.05-0.25 ng / mL, 2-4 mM of a lipid complex and trace elements with concentration of 1-5 mg / L. The serum-free animal-derived components are adopted, so that interference of many uncertain substances (such as different antigens, antibodies, hormone and cytokines) contained in serum on in-vitro expansion survival rate and the cell proliferation process of hematopoietic stemcells is eliminated, the proliferation efficiency is increased, the quantity of high-activity hematopoietic stem cells is increased, use types and adding content of the cytokines in the in-vitro expansion process of the hematopoietic stem cells are adjusted, then the activity of the hematopoietic stem cells in in-vitro expansion is improved, and death rate of the cells can be reduced.

Description

technical field [0001] The invention relates to the technical field of in vitro culture of stem cells, in particular to a serum-free culture medium for stem cells. Background technique [0002] Bone marrow hematopoietic stem cell and adult peripheral blood hematopoietic stem cell transplantation can treat hematological tumors (such as leukemia, multiple myeloma, etc.), aplastic anemia, immunodeficiency diseases and genetic diseases. In recent years, due to the advances in basic research on cord blood hematopoietic stem cells, it has been found that cord blood contains more abundant hematopoietic stem cells than bone marrow, and its ability to grow and expand is richer and easier to obtain than bone marrow. It is safe and easy to collect and less prone to virus transmission. and graft-versus-host disease. In the past, umbilical cord blood was a waste, but now, umbilical cord blood stem cells can be preserved through cord blood banks, which increases the possibility of its ra...

AI Technical Summary

Problems solved by technology

[0003] However, to make full use of umbilical cord blood stem cells, more methods are needed to increase the number of umbilical cord blood stem cells. At present, the most researched is the in vitro expansion of umbilical cord blood stem cells. However, human or animal serum is routinely added to the in vitro expansion, and serum contains Many uncertain substances (such as different antigens, antibodies, hormones and cytokines, etc.), in recent years, especially the virus infection in animals, the serum may carry some animal viruses, this is the use of serum to expand stem cells in vitro for transplantation In addition, the process of proliferation, differentiation, development and maturation of hematopoietic stem cells is quite complex and depends on the regulation of various hematopoietic factors, among which the role of cell stimulating factors is extremely critical, and related The composition and content of cell stimulating factors have a certain influence on the in vitro expansion of hematopoietic stem cells. Therefore, we propose a serum-free medium for stem cells