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C9orfll6 gene capable of regulating liver regeneration, and siRNA (Ribonucleic Acid) interference target spot and application thereof

A technology of liver regeneration and genetics, applied in the fields of molecular biology and biomedicine, can solve ambiguous problems

Inactive Publication Date: 2017-05-31
HENAN NORMAL UNIV +1
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Problems solved by technology

However, in rats, the c9orf116 gene is still a gene with unknown function, whether it can promote the proliferation of rat hepatocytes cultured in vitro is still unclear

Method used

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  • C9orfll6 gene capable of regulating liver regeneration, and siRNA (Ribonucleic Acid) interference target spot and application thereof
  • C9orfll6 gene capable of regulating liver regeneration, and siRNA (Ribonucleic Acid) interference target spot and application thereof
  • C9orfll6 gene capable of regulating liver regeneration, and siRNA (Ribonucleic Acid) interference target spot and application thereof

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Embodiment

[0020] BRL-3A cell culture: the rat BRL-3A liver cell line was purchased from the Cell Resource Center of the Institute of Basic Medical Sciences, Beijing Academy of Medical Sciences, and the medium was DMEM medium (Invitrogen Company), which contained 10% fetal bovine serum ( Hangzhou Tianhang Biotechnology Co., Ltd.) and 200U / mL penicillin and streptomycin (Invitrogen), at 37°C, with a volume fraction of 5% CO 2 and cultured cells under saturated humidity conditions.

[0021] siRNA sequence design and synthesis of c9orf116: Use Ambion, Qiagen, Dharmacon and other siRNA design software to obtain the rat c9orf116 mRNA sequence (NM_019165.1) according to GenBank to search for the AA sequence and record the 19 adjacent to the 3' end of each AA nucleotides to screen out siRNAs with a GC content between 30% and 55%. Further screening was carried out according to the basic design principles of siRNA, and the homology of the screened siRNA sequences was searched by BLAST in the gen...

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Abstract

The invention discloses a c9orf116 gene capable of regulating liver regeneration, and a siRNA (Ribonucleic Acid) interference target spot and application thereof, and belongs to the technical field of molecular biology and biological medicine. The technical scheme key point of the gene is the application of the antagonist of the c9orfll6 gene capable of regulating liver regeneration in preparing a medicine for accelerating liver regeneration. The nucleotide sequence of the c9orfll6 gene is disclosed in SEQ ID NO.1, the antagonist of the c9orf116 gene capable of regulating liver regeneration is the siRNA interference target spot expressed by a specificity interference c9orf116 gene, and the nucleotide sequence of the siRNA interference target spot is disclosed in SEQ ID No.2, SEQ ID NO.3 and SEQ ID NO.4. The c9orfll6 gene disclosed by the invention has a function for accelerating liver proliferation, the gene has an important function in a cell proliferation process, and the gene also has a potential application value on the aspects including cell proliferation and differentiation, tumor treatment, anti-tumor medicine research and development and the like.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and biomedicine, and specifically relates to a c9orf116 gene regulating liver regeneration and its siRNA interference target and application. Background technique [0002] The liver is an important organ of the body, which has the functions of storage, metabolism, biotransformation, detoxification, hematopoiesis, synthesis of bile pigment, secretion and regeneration. Studying the effect of liver regeneration-related genes on liver cell proliferation and liver regeneration has important theoretical significance and application value for revealing the mechanism of liver regeneration, constructing artificial liver, and establishing methods for treating and preventing liver diseases. At present, a large number of reports have pointed out that the application of RNAi technology to specifically introduce siRNA into mammalian and human cells to reduce the expression of target genes, thereby ca...

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Application Information

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IPC IPC(8): C12N15/12C07K14/47C07K16/18A61K48/00A61K45/00A61K31/713A61P1/16
Inventor 徐存拴张春艳孟竺张世馥常翠芳张全义史世会吕中原王绪洋
Owner HENAN NORMAL UNIV
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