The invention relates to a regadenoson purifying method and a new crystal form of regadenoson. The method comprises the following steps that a regadenoson crude product is heated and dissolved in a mixed solvent, and then stirring cooling, crystallization, filtering and drying are conducted, wherein the mixed solvent is selected from mixed solvents of ethanol, methanol, acetonitrile and water; orthe method comprises the following steps that the regadenoson crude product is heated and dissolved in a good solvent, then a poor solvent is added, and stirring cooling, crystallization, filtering and drying are conducted, wherein the good solvent is selected from DMF, NMP and sulfolane or a mixture of the DMF, NMP and sulfolane, and the poor solvent is selected from ethanol, methanol and water or a mixture of the ethanol, methanol and water.