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Rapid breeding method of Artemisia annua

An Artemisia annua, fast technology, applied in the field of plant biology, can solve the problems of long reproduction cycle, low reproduction coefficient, low rooting rate, etc., and achieve the effects of fast reproduction speed, stable genetics and low production cost

Inactive Publication Date: 2012-06-13
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can effectively maintain the genetic stability of Artemisia annua, but has the defects of long reproduction cycle and low efficiency
Chinese Invention Patent Publication No. CN101213915 discloses a method for asexual propagation of Artemisia annua with high content of artemisinin. This method uses side branch cuttings for asexual reproduction, which has the disadvantages of low rooting rate and low reproduction coefficient.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Cut 15-17cm young stems from a single plant of Artemisia annua that grows vigorously and is free from diseases and insect pests, put them upright in a glass container with a cover, add 0.5% sodium hypochlorite solution to cover the young stems, and drop Tween 20, so that the final concentration of Tween 20 is 0.05% (volume concentration), cover and soak for 20 minutes, shake the solution from time to time during the soaking process, after disinfection, pour out the disinfectant (sodium hypochlorite solution containing Tween 20), Rinse and sterilize the young stems with sterile water 4 times, and under aseptic conditions, cut the sterilized young stems into 1.3-1.7cm stem sections, each stem section has a bud head, and 300 bud heads are obtained. Stem segment, the stem segment cuttings are placed on the sterilized rooting medium, cultivated in the tissue culture room, the rooting medium is solid MS medium containing 0.08mg / L naphthaleneacetic acid (NAA), and the light con...

Embodiment 2

[0024] Cut 15-17cm long young stems from a single plant of Artemisia annua that grows vigorously and is free from diseases and insect pests, and put them upright in a glass container with a cover. Warm 20 to make the final concentration of Tween 20 to 0.05% (volume concentration), cover and soak for 18 minutes, shake the solution from time to time, after disinfection, pour out the disinfectant (sodium hypochlorite solution containing Tween 20), and use sterile Wash and sterilize the young stem 4 times with water, cut the young stem into 1.3-1.7cm stem segments under aseptic conditions, each stem segment has a bud, and obtain 260 stem segments with a single bud head, and the stem segment The cuttings were placed on the sterilized rooting medium and cultivated in the tissue culture room. The rooting medium was solid MS medium containing 0.02mg / LNAA. The light conditions were: 16h of light per day, 8h of darkness, light intensity of 2800lx, culture temperature After 10 days of cu...

Embodiment 3

[0026] Cut 15-17cm long young stems from a single Artemisia annua that grows vigorously and is free from diseases and insect pests, put them upright in a glass container with a cover, add sodium hypochlorite solution with a mass concentration of 1% to cover the young stems, and drop Tween 20, so that the final concentration of Tween 20 is 0.05% (volume concentration), cover the lid, soak for 15 minutes, shake the solution from time to time, after disinfection, pour out the disinfectant (sodium hypochlorite solution containing Tween 20), and use without Rinse and sterilize the young stems with sterile water 4 times, cut the young stems into 1.3-1.7cm stem segments under aseptic conditions, each stem segment has a bud, and obtain 230 stem segments with a single bud head. Segment cuttings were placed on sterilized rooting medium and cultivated in the tissue culture room. The rooting medium was solid MS medium containing 0.06 mg / LNAA. The light conditions were: 16 hours of light pe...

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Abstract

A rapid breeding method of Artemisia annua. Young stems on a single plant or a plurality of plants of Artemisia annua with completely consistent genotype are cut off; the young stems are disinfected in vitro and transversely cut into stem segments, and each stem segment has a bud; and the stem segments are cultured directly in a rooting medium and transplanted for soil culture after rooting. The method of the invention has easily available material, low variation rate, high increment coefficient, short rooting period, a two-week proliferation time reaching higher than 200 times, a rooting rate reaching higher than 90%, a transplanting survival rate reaching higher than 95% and strong factory production capability. Using the method of the invention to breed high-yield Artemisia annua linescan enable progeny to get stable heredity, so as to effectively reduce production cost of artemisinin.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a rapid asexual propagation method of Artemisia annua. [0002] Background technique [0003] Artemisia annua ( Artemisia annua L.), also known as Artemisia annua, Artemisia annua, is a plant of the genus Artemisia in the family Asteraceae, and is a traditional Chinese medicinal material in my country. Artemisinin is a sesquiterpene lactone peroxide isolated from Artemisia annua for the first time by Chinese scholars. It is a special drug for treating malaria, and the demand in the international market is increasing. Further pharmacological studies have proved that artemisinin and its analogs have toxic effects on a variety of tumor cells, including breast cancer cells, blood cancer cells, melanoma cells, kidney cancer cells, central nervous system tumor cells, lung cancer cells, prostate cancer cells Etc., but little damage to normal cells, and there is no cross...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘硕谦刘仲华田娜黄建安
Owner HUNAN AGRICULTURAL UNIV
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