Rotary pouring type bioreactor system

Abstract

The invention discloses a rotating and irrigating type bioreactor system which employs the combination of rotating and irrigating to make the cells distribute more uniformly on the stent surface, promote the transportations of oxygen and nutritious substances into the inner microholes of the stent, keep the activities of the inner cells of the stent. The invention can produce mechanical stress stimulates to the cells by the flow of the culture liquid, regulate the exertion of the cell functions, and realize the real time exchange of the inside and outside gas of the reactor through the semi-permeable membrane on the two ends of the reactor. The invention can provide an excellent, controllable three-dimensional environment for the cell implantation and in vitro culture of cell / stent structural body to realize genuine three-dimensional dynamic culture.

Description

technical field [0001] The invention belongs to the field of biological equipment manufacturing engineering, and relates to a bioreactor system for cell culture, in particular to a rotary perfusion bioreactor system applied to biomedical engineering and tissue engineering cell culture. Background technique [0002] The bioreactor system is a three-dimensional dynamic cell culture system, which overcomes many shortcomings of static culture, such as uneven distribution of oxygen and nutrients, and can only provide cells with oxygen and nutrients by diffusion, and the number of cells cultured limited. The main advantages of this dynamic culture system are: (1) effective and uniform cell planting can be produced in the three-dimensional scaffold, and the number of cells is large; (2) it can promote the delivery of oxygen and nutrients to the interior of the scaffold, and maintain the The activity and function of the cells (3) can promote the proliferation and differentiation of...

AI Technical Summary

Problems solved by technology

The disadvantage is that the culture environment of the micropores or microchannels inside the scaffold has not been improved, so that the function of the cells attached to the micropores or microchannels is limited, and the number of cells must be limited.

The perfusion bioreactor makes up for the shortcomings of the rotary bioreactor. The peristaltic pump is used to continuously perfuse the culture solution into the micropores or micropipes inside the scaffold, so that the culture environment in the micropores or micropipes is well maintained. Improve and promote the function of cells, but the cells on the surface of the scaffold are difficult to grow

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