Rotary pouring type bioreactor system

A bioreactor and perfusion technology, which is applied in biochemical instruments, biochemical equipment and methods, tissue cell/virus culture devices, etc., can solve the problems of cell function limitation, limited number of cells, and difficult growth of cells, etc., to achieve The effect of maintaining activity, better and uniform distribution, and basically stable content

Inactive Publication Date: 2005-07-06
XI AN JIAOTONG UNIV
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AI Technical Summary

Problems solved by technology

The disadvantage is that the culture environment of the micropores or microchannels inside the scaffold has not been improved, so that the function of the cells attached to the micropores or microchannels is limited, and the number of cells must be limited.
The perfusion bioreactor makes up for the shortcomings of the rotary bioreactor. The peristaltic pump is used to continuously perfuse the culture solution into the micropores or micropipes inside the scaffold, so that the culture environment in the micropores or micropipes is well maintained. Improve and promote the function of cells, but the cells on the surface of the scaffold are difficult to grow

Method used

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  • Rotary pouring type bioreactor system
  • Rotary pouring type bioreactor system
  • Rotary pouring type bioreactor system

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Embodiment Construction

[0017] see figure 1 , the rotary perfusion bioreactor system of the present invention comprises:

[0018] A permanent magnet DC gear reduction motor 19, used to drive and control the rotation of the bioreactor;

[0019] The perfusion system is used to provide the culture medium in the bioreactor, and the perfusion system includes a peristaltic pump 18 and pump tubes 23 of various inner diameters matched with the peristaltic pump;

[0020] Bioreactor, this bioreactor comprises base 1 and support 2, and reactor vessel 12 is arranged on support 2 through bearing 6, and medical tee 20 is arranged on the reactor vessel; Bushing 10, right bushing 15, left end cover 9, right end cover 16, bearing retaining ring 5, dynamic sealing ring 4, rotary joint 3, four fan-shaped grooves are respectively opened on the left and right bushings and the left and right end covers 24. The fan-shaped groove is pasted with a semi-permeable membrane 17; the semi-permeable membrane is used for gas exch...

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Abstract

The invention discloses a rotating and irrigating type bioreactor system which employs the combination of rotating and irrigating to make the cells distribute more uniformly on the stent surface, promote the transportations of oxygen and nutritious substances into the inner microholes of the stent, keep the activities of the inner cells of the stent. The invention can produce mechanical stress stimulates to the cells by the flow of the culture liquid, regulate the exertion of the cell functions, and realize the real time exchange of the inside and outside gas of the reactor through the semi-permeable membrane on the two ends of the reactor. The invention can provide an excellent, controllable three-dimensional environment for the cell implantation and in vitro culture of cell/stent structural body to realize genuine three-dimensional dynamic culture.

Description

technical field [0001] The invention belongs to the field of biological equipment manufacturing engineering, and relates to a bioreactor system for cell culture, in particular to a rotary perfusion bioreactor system applied to biomedical engineering and tissue engineering cell culture. Background technique [0002] The bioreactor system is a three-dimensional dynamic cell culture system, which overcomes many shortcomings of static culture, such as uneven distribution of oxygen and nutrients, and can only provide cells with oxygen and nutrients by diffusion, and the number of cells cultured limited. The main advantages of this dynamic culture system are: (1) effective and uniform cell planting can be produced in the three-dimensional scaffold, and the number of cells is large; (2) it can promote the delivery of oxygen and nutrients to the interior of the scaffold, and maintain the The activity and function of the cells (3) can promote the proliferation and differentiation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 李涤尘李祥卢秉恒王臻王林
Owner XI AN JIAOTONG UNIV
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