Sinkiang drug mulberry tissue culture and rapid propagation culture medium

A technology of tissue culture rapid propagation and culture medium, which is applied in the field of plant tissue culture, and can solve the problems of difficult survival and poor affinity

Inactive Publication Date: 2013-09-04
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the compatibility between Xinjiang medicine mulberry and the diploid mulberry tree used as rootstock is poor, and it is not easy t...

Method used

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  • Sinkiang drug mulberry tissue culture and rapid propagation culture medium
  • Sinkiang drug mulberry tissue culture and rapid propagation culture medium
  • Sinkiang drug mulberry tissue culture and rapid propagation culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In October, take the annual branches (20-25cm) of Xinjiang Yaosang with winter buds, cut healthy and full winter buds, peel off the base and outer scales of the buds, wash the surface of the buds, rinse with running water for 30-60 minutes, and place them on a clean bench , 75% alcohol disinfection for 30s, rinse with sterile water 3 times, and then use 0.1% HgCl 2 Sterilize the solution for 10 minutes, rinse with sterile water for 5 times, peel off the surface scales on the inoculation plate to expose the green buds, inoculate it on the germination medium, grow at 24℃±2℃, and light intensity 30μEm -2 s -1 Cultivate for 2-3 weeks ( figure 1 ), the illumination time is 14 hours / day. After 2-3 weeks, the germinated young shoots are cut off and placed on a proliferation medium for subculture. The culture conditions are the same as the primary generation. After 15 days, the lateral buds germinate in large quantities, and the seedling height is 2.5-4.0cm after 25-30 days ( fig...

Embodiment 2

[0035] In March, take the Xinjiang medicine mulberry seedlings that were inherited in February, cut the callus at the base of the seedlings to disperse them into individual plants, and inoculate the main stem (greater than 4.0cm) on the extension medium, and the lateral branches (1.0-3.0cm) first Inoculated on the propagation medium ( image 3 ), grow at 24℃±2℃, light intensity 30μEm -2 s -1 After cultivating for one month, transfer to the extension medium. When the seedlings are 5-8cm high, the plants are growing well, cut off the callus at the base of the seedlings and transfer the individual plants to the rooting medium for culture. New roots will germinate after 10 days and the base of the seedlings after 30 days. Generate a lot of new roots.

[0036] The tissue cultured seedlings of Xinjiang mulberry with more than 4 new roots and root length greater than 5cm are removed from the culture room, and the culture bottle and root culture medium are washed thoroughly after uncover...

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Abstract

A set of Sinkiang drug mulberry tissue culture and rapid propagation culture medium comprises the following components: a germination culture medium, a multiplication culture medium, an extension culture medium and a root culture medium, wherein the germination culture medium is prepared from the following raw materials: MS 4.33 g/L, 6-BA 2.0 mg/L, NAA 0.2 mg/L, sugar 30 g/L and agar 7 g/L, and the pH value of the germination culture medium is 5.8; the multiplication culture medium is prepared from the following raw materials: MS 4.33 g/L, 6-BA 1.0 mg/L, sugar 30 g/L and agar 7 g/L, and the pH value of the multiplication culture medium is 5.8; the extension culture medium is prepared from the following raw materials: MS 4.33 g/L, ZT 4.5 micro-mol/L, sugar 30 g/L and agar 7 g/L, and the pH value of the extension culture medium is 5.8; and the root culture medium is prepared from the following raw materials: WPS 2.30 g/L, L+NAA 0.2 mg/L, IBA 0.2 mg/L, activated carbon 0.4 g/L, sugar 20 g/L and agar 6 g/L, and the pH value of the root culture medium is 6.0. The culture medium is simple and convenient to prepare, and is high in multiplication efficiency, each seedling can differentiate 2.3 lateral buds averagely, the transplanted seedlings are got by winter buds after four months, and the survival rate after transplantation is high to more than 75%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a set of Xinjiang medicinal mulberry tissue culture rapid propagation medium. Background technique [0002] Xinjiang medicine mulberry belongs to Moraceae, Morus, and black mulberry species (Morus nigra Linn.) in plant taxonomy. It was introduced into my country from western Iran in the 16th century and is mainly cultivated in Hotan, Kashgar, Aksu and other vast areas in southern Xinjiang. It is an important medicinal mulberry germplasm resources. Xinjiang medicine mulberry is a deciduous tree with a height of 3-7m or higher and a larger crown. The germination period is mid-to-late April, and the leaf-opening period is early May; the ripening period of mulberries is from mid-to-late June to mid-to-late August, and the fruit shedding period is in late August. After ripening, the fruit is purple-black or purple-red, sour Sweet and delicious, with a high juice yield. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何宁佳曾其伟王青王旭伟赵爱春向仲怀
Owner SOUTHWEST UNIVERSITY
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