Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant

An embryogenic cell and plant regeneration technology, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of long cycle, low proliferation rate, complex plant regeneration steps, etc., and achieve shortened time and simplified operation. effect of the process

Active Publication Date: 2013-01-30
广东省农业科学院花卉研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the low proliferation rate of embryogenic cell suspension culture system in the prior art, complicated plant regeneration steps and long cycle, and provide an improved method for fast proliferation of anthurium embryogenic cells and regeneration of plants

Method used

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  • Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant
  • Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant

Examples

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Effect test

Embodiment 1

[0028] (1) Embryogenic induction of embryogenic callus: Take young leaves of young seedlings of Anthurium cultivar Alabama, after surface disinfection treatment, cut the leaves into 5 mm fragments, and inoculate them on embryogenic callus induction medium cultured in a dark environment at a temperature of 26°C for 4 weeks to obtain yellow embryogenic callus;

[0029] (2) Establishment of embryogenic cell suspension culture line: In a metal mesh with a pore size of 1.0 mm, the embryogenic callus was broken with tweezers, and sieved through the metal mesh to form embryogenic cell clusters with a diameter of no more than 1.0 mm; 0.1 g The embryogenic cell mass was inoculated in a 100ml Erlenmeyer flask with 20ml of embryogenic cell suspension medium, and cultured at a temperature of 26°C, with light of 900 lux, and shaking at 100 rpm; when subcultured, the proliferated The embryogenic cell mass was broken, and 0.1 g of the embryogenic cell mass was transferred to a 100 ml Erlenme...

Embodiment 2

[0035] The difference between this example and Example 1 is that the stems of the young seedlings of the anthurium cultivar 'Alabama' are treated with surface disinfection, cut into 10 mm sections, and then inoculated in somatic embryo induction medium to cultivate the obtained body. Cell embryo, all the other are identical with embodiment 1. Under this condition, the embryogenic callus induction rate of Anthurium anthurium reached 95.1%.

Embodiment 3

[0037] The difference between this example and Example 1 or 2 is that the embryogenic cell suspension medium in step (2) contains 2.0 mg / L of 6-BA and 2.0 mg / L of 2,4-D, and the rest are the same as in Example 1 or 2 for the same. Under this condition, the suspension culture of embryogenic cells can proliferate 2.82 times per week and more than 63 times per month.

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Abstract

The invention discloses a method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate a plant. By changing medium components, light condition and subculture interval of suspension culture in the prior art, an embryogenic cell cluster can proliferate 2.82-3.02 times per week and proliferate 83 times per month, the proliferation efficiency is improved by more than 15 times compared with that in the prior art, the plant regeneration operating process is simplified, and the plant regeneration of the embryogenic cell cluster can be completed by only one step; meanwhile, the plant regeneration time is shortened, and is 6-10 weeks, which is over 20 days shorter than that in the prior art.

Description

technical field [0001] The invention relates to the technical field of plant induced regeneration, in particular to a method for rapidly proliferating anthurium embryogenic cells and regenerating plants. Background technique [0002] Anthurium ( Anthurium andraeanum ), also known as Anthurium and Anthurium, is an anthurium plant of the Araceae family and is an important tropical ornamental plant. Anthurium is the main source of cut flowers and potted flowers in many tropical countries and regions, and has developed into the second largest commodity of tropical flowers after tropical orchid. At present, the production of anthurium seedlings mainly adopts the organogenesis pathway, which has disadvantages such as a long callus induction period, and the aseptic seedlings are prone to degeneration and variation, while the somatic embryogenesis pathway has the advantages of large number of somatic embryos and fast reproduction speed. , genetic stability and other advantages, co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 于波廖飞雄刘金梅刘晓荣朱根发操君喜李伟峰
Owner 广东省农业科学院花卉研究所
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