Method of tissue culture and rapid propagation of healthy sugarcane seedlings

Abstract

A method of tissue culture and rapid propagation of healthy sugarcane seedlings comprises: detoxifying a small amount of cleaned sugarcane mature seed rhizomes with warm of 52 DEG C water for 30 minutes, accelerating germination at 38 DEG C, using auxiliary buds as explants for induction, differentiation and breeding to obtain first generation healthy seedlings, detecting ratoon stunting diseasesby means of PCR (polymerase chain reaction) to detect plantlets with negative results, obtaining a great amount of healthy sugarcane seedlings by means of multiplication culture, performing rooting cultivation, seedling adaptation and transplanting to a sand bed for temporary planting, sampling the seedlings for detecting the ratoon stunting diseases by means of PCR, proving qualification of thehealthy sugarcane seedlings, and transplanting the qualified seedlings to a large land or popularizing planting of the seedlings. The method can improve production efficiency of healthy sugarcane seedlings, twice PCR detection guarantees detoxification effect of the healthy sugarcane seedlings, the whole production process is short, propagation by multiple nursery is not needed, seedlings from tissue cultivation are directly used for production or application, the method has the advantages of high efficiency, superior quality, fastness and the like and meets large-scale factory production requirements of the healthy sugarcane seedlings.

Description

Technical field

[0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of healthy sugarcane seedlings. Background technique

[0002] sugar cane( Saccharum.ssp ) Is the most important sugar crop in my country and the most important energy plant in the world. The rapid promotion and application of high-quality new varieties of sugarcane has become a new driving force for my country's sugarcane sugar industry. However, sugarcane ratoon stunting disease (RSD) has caused serious damage in my country’s main sugarcane producing areas, resulting in some varieties of my country. Sexual degradation has seriously affected the quality and yield of sugarcane, and brought great losses to my country's sugar industry. At present, there is no master variety or new variety that can resist sugarcane ratoon stunting disease, so the best control method is to promote the cultivation of healthy sugarcane seedlings...