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Immunochemical dyeing kit for auxiliary diagnosis of cervical cancer

A technology of reagents and surfactants, which is applied in the field of liquid-based cytology and immunocytochemistry, can solve the problems of missed diagnosis, false positives, and false negatives of high-risk patients, and achieve the effects of saving operation time, easy storage, and fast sealing speed

Active Publication Date: 2019-06-07
肖国林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, limited by the experience of pathologists, there are certain false positives and false negatives, resulting in the missed diagnosis of some high-risk patients.

Method used

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  • Immunochemical dyeing kit for auxiliary diagnosis of cervical cancer
  • Immunochemical dyeing kit for auxiliary diagnosis of cervical cancer
  • Immunochemical dyeing kit for auxiliary diagnosis of cervical cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Provides an immunochemical staining kit for auxiliary diagnosis of cervical cancer, including ready-to-use mixed primary antibody working solution (1 bottle), ready-to-use mixed secondary antibody working solution (1 bottle), rapid protein-free blocking solution ( 1 bottle), stable horseradish peroxidase chromogenic reagent (3 bottles), ready-to-use alkaline phosphatase chromogenic reagent (1 bottle), aqueous mounting medium (1 bottle).

[0076] The ready-to-use mixed primary antibody working solution includes PBS buffer solution with a pH value of 7.4, p16 INK4a Monoclonal antibody, Ki-67 monoclonal antibody, bovine serum albumin, trehalose, betaine, β-cyclodextrin, sodium chloride, Tween-20, KATHON; wherein, the p16 INK4aThe antibody concentration is 0.2mg / L; the Ki-67 antibody concentration is 0.3mg / L; the bovine serum albumin concentration is 0.5g / L; the trehalose concentration is 1.7g / L; the betaine concentration It is 3.6g / L; the β-cyclodextrin concen...

Embodiment 2

[0089] Example 2 Provides an immunochemical staining kit for auxiliary diagnosis of cervical cancer, including ready-to-use mixed primary antibody working solution (1 bottle), ready-to-use mixed secondary antibody working solution (1 bottle), rapid protein-free blocking solution ( 1 bottle), stable horseradish peroxidase chromogenic reagent (3 bottles), ready-to-use alkaline phosphatase chromogenic reagent (1 bottle), aqueous mounting medium (1 bottle).

[0090] The ready-to-use mixed primary antibody working solution includes PBS buffer solution with a pH value of 7.2, p16 INK4a Monoclonal antibody, Ki-67 monoclonal antibody, bovine serum albumin, trehalose, betaine, β-cyclodextrin, sodium chloride, Tween-20, KATHON; wherein, the p16 INK4a The antibody concentration is 0.3mg / L; the Ki-67 antibody concentration is 0.3mg / L; the bovine serum albumin concentration is 0.4g / L; the trehalose concentration is 2g / L; the betaine concentration is 2g / L; the concentration of β-cyclodex...

Embodiment 3

[0103] Example 3 Provides an immunochemical staining kit for auxiliary diagnosis of cervical cancer, including ready-to-use mixed primary antibody working solution (1 bottle), ready-to-use mixed secondary antibody working solution (1 bottle), rapid protein-free blocking solution ( 1 bottle), stable horseradish peroxidase chromogenic reagent (3 bottles), ready-to-use alkaline phosphatase chromogenic reagent (1 bottle), aqueous mounting medium (1 bottle).

[0104] The ready-to-use mixed primary antibody working solution includes PBS buffer solution with a pH value of 7.7, p16 INK4a Monoclonal antibody, Ki-67 monoclonal antibody, bovine serum albumin, trehalose, betaine, β-cyclodextrin, sodium chloride, Tween-20, KATHON; wherein, the p16 INK4a The antibody concentration is 1.2mg / L; the Ki-67 antibody concentration is 1.1mg / L; the bovine serum albumin concentration is 2.2g / L; the trehalose concentration is 7g / L; the betaine concentration is 4g / L; the concentration of β-cyclodex...

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Abstract

The invention relates to the field of liquid-based cytology immunocytochemistry, in particular to an immunohistochemical kit for auxiliary diagnosis of cervical cancer. Ready-to-use antibody working liquid is provided and prepared from an antibody reagent, bovine serum albumin, non-reductive sugar, betaine, cyclodextrin, a neutral inorganic salt, a surfactant and a preservative, wherein the antibody reagent is a reagent A or a reagent B; the reagent A is a phosphate buffer solution (PBS) of a p16<INK4a> monoclonal antibody and a Ki-67 monoclonal antibody; the reagent B is a PBS of horse radishperoxidase conjugated GaMIgG and an alkaline phosphatase conjugated goat anti-rabbit secondary antibody; and the pH value of the PBSs is 7.2-7.8.

Description

technical field [0001] The invention relates to the field of liquid-based cytology immunocytochemistry, in particular to an immunochemical staining kit for auxiliary diagnosis of cervical cancer. Background technique [0002] Cervical cancer is one of the most common malignant tumors in gynecology. Its incidence is second only to breast cancer and tends to be younger, seriously endangering women's health. However, cervical cancer is the only clear cause, and it is expected to become the first tumor to be conquered by humans. Therefore, prevention and early screening are of great significance to reduce the incidence and mortality of cervical cancer. [0003] At present, the main methods of cervical cancer diagnosis are: cervical exfoliation cytology examination, human papillomavirus (HPV) detection, colposcopy, pathological biopsy, etc.; the three-stage procedure for cervical cancer diagnosis recommended by the Chinese Medical Association is cytology, colposcopy, etc. , Cer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N33/574G01N33/535G01N33/577G01N33/58
Inventor 肖国林
Owner 肖国林
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