Simplified thin-layer liquid-based cytology cell smeard preparation method

Abstract

The invention discloses a simple thin liquid-based cytology examine method applied for the gynecological examination, the cervical cancer general examine and the cervical cancer preceding lesion of selected crowd, the steps including: using the cervical cells brush and the scraper to collect samples, put samples in the liquid-based cell preservation solution, add base liquid cell processing solution, oscillate, centrifugally separate, take and discard the supernatant samples liquid, using adding samples gun to assimilate sediment samples, smear, sheet drying, using 95% ethanol marinate or spray for fixed, washing and dyeing. The invention has simple method, small steps, high positive detection rate, low misdiagnosis rate, low cost, and it can effectively eliminate the red blood cells, mucus and other interference components from the cervical cytology detection specimens, with good sheet-producing result.

Description

technical field

[0001] The invention relates to a cytological examination method, in particular to a simple thin-layer liquid-based cytological examination method that can be used for gynecological physical examination, cervical cancer screening and screening of cervical precancerous lesions in crowds. Background technique

[0002] There are 500,000 new cases of cervical cancer every year in the world, and China accounts for 1 / 4 and shows an upward and younger trend. Cervical cancer is the only malignant tumor in the human body that can be diagnosed early by screening, find out precancerous lesions (intraepithelial neoplasia), and be 100% cured. Therefore, it is of great clinical significance and application value to develop a thin-layer liquid-based cytology examination method with simple method, low cost, high accuracy, specificity and sensitivity for general screening and physical examination.

[0003] The traditional ordinary Pap smear has a 15% to 20% missed diagnosis ...