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Simple and quick preparation method of thin-layer liquid-based cytology cell smear

A cell smear and cytology technology, applied in the field of cytology examination, can solve the inconvenience and other problems, achieve the effect of simple equipment, convenient operation method, and improve the accuracy of film reading

Inactive Publication Date: 2012-03-28
张雪云 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, there are above-mentioned inconveniences and problems in the known thin-layer liquid-based cytology cell smear preparation method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Method for making thin-layer liquid-based cytology cell smears from gynecological specimens

[0035] a. Specimen pretreatment

[0036] Put the gynecological specimen (including the preservation bottle) on the shaker for 5 minutes, absorb 10 ml of the middle and bottom cells in the preservation bottle, mix well and take 5 ml as a sample; the liquid-based cell preservation solution contains formaldehyde and ethanol that can fix cells , preservative sodium diacetate, anticoagulant sodium citrate, buffering agent sodium chloride, potassium chloride and sodium bicarbonate, chelating agent EDTA and glacial acetic acid to dissolve red blood cells.

[0037] b. Take an ordinary centrifuge tube and add 5 ml of adhesion separation solution, then slowly add the same amount of sample pretreated in step a along the wall of the centrifuge tube; the adhesion separation solution contains ethanol, sodium chloride, EDTA, sodium bicarbonate and glacial acetic acid.

[0038] c. Place the ...

Embodiment 2

[0047] Method for making thin-layer liquid-based cytology cell smears from gynecological specimens

[0048] a. Specimen pretreatment

[0049]Put the gynecological specimen (including the preservation bottle) on the shaker for 5 minutes, let the specimen stand for a while, discard the supernatant, leave the bottom 10 ml, mix well and take 5 ml as a sample; liquid-based cell preservation solution contains fixed cells The role of formaldehyde and ethanol, antiseptic sodium diacetate, anticoagulant sodium citrate, buffering agent sodium chloride, potassium chloride and sodium bicarbonate, chelating agent EDTA and glacial acetic acid to dissolve red blood cells. The amount of specimens used in gynecology is small, and the preservation solution is generally 15-20 ml.

[0050] b. Take an ordinary centrifuge tube and add 5 ml of adhesion separation solution, then slowly add the same amount of sample pretreated in step a along the wall of the centrifuge tube; the adhesion separation s...

Embodiment 3

[0060] Method for making thin-layer liquid-based cytology cell smears from gynecological specimens

[0061] a. Specimen pretreatment

[0062] Put the gynecological specimen (including the preservation bottle) on the shaker for 10 minutes, mix well and take 10 ml as a sample; the liquid-based cell preservation solution contains formaldehyde and ethanol that can fix cells, preservative sodium diacetate, anticoagulant citrate Sodium citrate, buffering agents sodium chloride, potassium chloride and sodium bicarbonate, chelating agent EDTA and glacial acetic acid to dissolve red blood cells.

[0063] b. Take an ordinary centrifuge tube and add 5 ml of adhesion separation solution, then slowly add the same amount of sample pretreated in step a along the wall of the centrifuge tube; the adhesion separation solution contains ethanol, sodium chloride, EDTA, sodium bicarbonate and glacial acetic acid.

[0064] c. Place the sample mixture in step b in a centrifuge at 2000 rpm for 5 min...

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PUM

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Abstract

The invention discloses a simple and quick preparation method of a thin-layer liquid-based cytology cell smear. The method is characterized by comprising the following steps: a, preprocessing a specimen; b, adding 5ml of adhesive separation liquid into a common centrifuge tube; c, centrifuging a sample mixture and discharging the precipitation from the centrifuge tube while reserving the supernate for use; d, placing a glass sheet with a written number into a long slot of a natural settling device fixing seat serving as corollary equipment for film making; e, placing a settling tube on the glass sheet; f, uniformly mixing the bottom sample left after the centrifugation of the step c, and adding the mixture into the settling tube; g, standing still and naturally settling for 15-30 minutes; h, rinsing the specimen with a washing liquid; i, fixing in 95% alcohol for 10 minutes, and performing Papanicolaou staining; and H, performing E staining or immunohistochemical staining and reading the film. Through the simple and quick preparation method of the thin-layer liquid-based cytology cell smear disclosed by the invention, the mucus, blood and inflammatory cells in the specimen can be separated by natural precipitation, the film is clear and the diagnosis accuracy is improved.

Description

technical field [0001] The invention relates to a cytological examination method, specifically, a cytological examination method for pleural effusion, urine, sputum, bronchial brush examination specimens, gynecological physical examination, cervical cancer screening and screening of cervical precancerous lesions in the crowd, etc. A simple and rapid thin-layer liquid-based cytology cell smear preparation method. Background technique [0002] Liquid-based thin-layer cell detection technology is an advanced cervical cancer cell detection technology in the world today. It has changed the method of collecting cells in conventional cervical smears. Immediately after the specimen is collected, it is placed in the cell preservation solution. After automatic cell production After centrifugation with the specific gravity liquid, the mucus, red blood cells, and inflammatory cells in the preservation solution are separated, and the remaining cervical epithelial cells are collected to m...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 张雪云张一心张一意
Owner 张雪云
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