Space enterococcus faecium LCT (Liquid-based Cytology Test )-EF301

A technology of LCT-EF301, Enterococcus faecium, applied in the field of microorganisms, can solve the problems that have not been reported, limitations and other issues.

Inactive Publication Date: 2013-05-29
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Previous studies on the biological characteristics and uses of Enterococcus faecium were

Method used

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  • Space enterococcus faecium LCT (Liquid-based Cytology Test )-EF301

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, hemolysis test of Enterococcus faecium LCT-E F301

[0019] The hemolysis test adopts spotting method. The strains are spot-planted on the blood agar medium, kept at a constant temperature of 37°C, and cultured upside down. As a result, there is no hemolysis pattern around the colony of Enterococcus faecium LCT-E F301, see figure 1 . It can be seen from the figure that Enterococcus faecium LCT-EF301 has no hemolysis phenomenon.

Embodiment 2

[0020] Embodiment 2, the biolog test of Enterococcus faecium LCT-EF301

[0021] 1) Bacterial suspension preparation: Take out the slant surfaces of the space strain Enterococcus faecium LCT-E F301 and the ground control strain, dip the bacteria with a sterilized bamboo stick and inoculate them in LB liquid medium (4ml system) for 24 hours. Then take 2ml of bacterial liquid at 6000r / s, centrifuge for 5min, discard the supernatant medium, and leave the centrifuged bacterial cells. Wash the cells with sterilized normal saline, centrifuge at 6000r / s for 5min, discard the supernatant again, suspend the cells with Biolog inoculum, and finally transfer to 15ml inoculum to adjust the concentration to about 107-108.

[0022] 2) Inoculation: Pour the above bacterial suspension into the inoculation dish, oscillate evenly, add the sample with a 12-channel (or 8-channel) row gun, add 100ul to each Biolog plate hole, a total of 96 wells, after adding the sample, cover Cover the Biolog plat...

Embodiment 3

[0025] Example 3. Genome, transcriptome sequencing and proteomic analysis of Enterococcus faecium LCT-EF301

[0026] The whole genome sequence of Enterococcus faecium LCT-EF301 was performed using high-throughput Illumina sequencing technology. The whole genome sequence of Enterococcus faecium LCT-EF301 includes 14 Scaffolds. The whole genome sequence is 2,696,027bp long and has 1 SNP site. type, with a GC content of 37.98%, and 2,639 potential protein-coding genes.

[0027] After extracting the total RNA of Enterococcus faecium LCT-EF301 strain, use the kit to remove the rRNA, add fragmentation buffer to break the mRNA into short fragments, and use the mRNA as a template to synthesize the first cDNA strand with six base random primers (random hexamers) , then add buffer, dNTPs, RNase H and DNA polymerase l to synthesize the second cDNA strand, after purification by QiaQuick PCR kit and elution with EB buffer, do end repair, add polyA and connect the sequencing adapter, and th...

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Abstract

The invention discloses space enterococcus faecium LCT (Liquid-based Cytology Test)-EF301, in particular relates to space microorganism and discloses enterococcus probiotics which is obtained by space technology. The space enterococcus faecium LCT-EF301 is characterized in that a gram-positive bacterium is a lactic acid bacteria which is facultative anaerobic, described in details from genomics, transcriptomics and proteomics, free of toxicity. The space enterococcus faecium LCT-EF301 disclosed by the invention can be used for generating bacteriocin for restraining growth of other bacteria, generating acid materials (see figure 1, figure 1a being LCT-EF301 and figure 1b being a control group) such as lactic acid and the like by fermenting carbon sources such as glucose and the like through a high-efficiency glycolysis way, keeping the acid environment of the intestinal tract, restraining the growth of the pathogenic bacteria, adjusting the ecological balance of the intestinal flora of spaceman and producing medicines and health-care products including acidified milk food.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a space bacterial strain Enterococcus faecium LCT-EF301. The invention relates to a preparation method of the space bacterial strain, strain characteristics and uses thereof. Background technique [0002] With the continuous improvement of my country's space science and technology level, our country's space exploration activities are becoming more and more frequent. It is expected that a space station will be built in 2020 and will be equipped with astronauts stationed for a long time. The health protection of astronauts is a crucial issue. There are special environmental factors such as strong radiation, microgravity, high vacuum, extreme temperature, and weak magnetic field in space. Studies have confirmed that the space environment has an impact on human bones, blood circulation, and immune systems, as well as the bacteria that inhabit the human body. Space radiation includes parti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P1/00A23L1/29A23C9/12C12R1/01A23L33/00A61K35/744
Inventor 刘长庭苏龙翔陈振鸿方向群李天志王俊峰郭英华常德王亚娟郭娜
Owner GENERAL HOSPITAL OF PLA
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