The invention discloses a pathological picture three-dimensional reconstruction method in the technical field of pathological picture display. The method comprises the following steps: S1, fixing a tumor specimen; S2, dehydrating the tumor specimen; S3, transparentizing the tumor specimen; S4, performing wax dipping on the tumor specimen; S5, embedding the tumor specimen; S6, carrying out linear bio-labeling on rows; S7, performing continuous slicing on rows; S8, carrying out HE dyeing and flaking; S9, performing immunohistochemical staining and flaking; S10, performing molecular biological detection and flaking; S11, observing with a microscope; and S12, synthesizing a three-dimensional picture. According to the method, on the basis of the two-dimensional pictures, continuous slicing and accurate positioning of the pathological specimen are carried out, so that after the tumor leaves the body, three-dimensional reconstruction of a plurality of fault pictures and three-dimensional reconstruction of the pathological picture are finally realized through sequential listing, proofreading and accurate positioning of the two-dimensional pictures in a pathological space; pathologists can observe more complete tumor tissue images as much as possible, and more complete and accurate pathology reports are provided for patients and clinicians.