Liquid-based cytology detection method

Abstract

A purpose of the invention is to disclose a liquid-based cytology detection method, which comprises: 1, numbering selected bloody and mucus specimens according to centrifugal tubes, wherein a group comprises eight specimens; 2, placing the specimens in the steps 1 into a centrifuge, and carrying out centrifugation for 5 min at 2500 r / min; 3, pouring the upper layer storage clarification liquid obtained after the centrifugation into a corresponding specimen storage bottle, adding 30 ml of 10% glacial acetic acid to downwardly precipitated cell mass, mucus mass and blood cell mass, covering with a lid, placing into a shaker, and carrying out oscillation for 8-10 min; 4, carrying out centrifugation on the oscillated product obtained in the step 3 for 5 min at 2500 r / min, removing the upper layer mixing liquid after the centrifugation, mixing the lower layer cell mass and a corresponding storage liquid, and pouring the obtained mixture into a storage bottle; 5, producing a liquid-based thin-layer cell sheet gynecological specimen from the product obtained in the step 4; and 6, staining the liquid-based thin-layer cell sheet obtained in the step 5, carrying out sealed fixation, and carrying out air drying. The liquid-based cytology detection method of the present invention has advantages of simple, convenient and flexible operation and substantially improved detection accuracy.

Description

technical field [0001] The invention belongs to cytological detection technology, and specifically refers to a liquid-based cytological detection method. Background technique [0002] Gynecological morbidity remains high and mortality is high. For example, cervical cancer has an incidence rate as high as 81 / 100,000 in some backward areas that lack reasonable screening. According to preliminary estimates, by 2030, there will be approximately 474,000 cervical cancer deaths per year, of which more than 95% may occur in backward and developing countries. Therefore, it is of great significance to find an effective method for early screening of cancer, to screen out cervical precancerous lesions and tumor patients, and to implement effective early diagnosis and treatment. [0003] At present, my country adopts a screening strategy for gynecological diseases with liquid-based cytology as the main content, and at the same time, combined screening with HPV-DNA+cytology is used loca...

AI Technical Summary

Problems solved by technology

[0006] The main problem solved by this application is to provide a liquid-based cytology detection method, which not only has the advantages of simple operation of conventional liquid-based cytology detection methods, but also has a more scientific and standardized operation process and greatly improves the detection accuracy of liquid-based cytology A detection method to solve the technical problems of a liquid-based cytology detection method, such as high cost, difficult operation, difficult to replicate operation, and high failure rate of specimens