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Liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus

A human papillomavirus and cytology technology, applied in the field of joint cervical cancer screening, can solve the problems of complicated operation and inconsistent samples, and achieve the effect of ensuring uniformity, improving accuracy and improving efficiency.

Active Publication Date: 2021-10-08
武汉兰丁云医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the sample is collected twice at the same time, it is easy to have the problem of inconsistent samples, and the operation is also very cumbersome. At present, there is no better solution

Method used

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  • Liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus
  • Liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus
  • Liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Such as figure 1 , 2 Among them, a liquid-based cytology and human papillomavirus combined screening device for cervical cancer, which includes a sealing cap 2, and the sealing cap 2 is used for sealing connection with the bottle mouth of the elution bottle 1;

[0030] An air inlet pipe 23 is arranged on the sealing cover 2, and one end of the air inlet pipe 23 communicates with the inner cavity of the elution bottle 1, and the other end of the air inlet pipe 23 is connected with the air bag 3;

[0031] The sealing cover 2 is also provided with a liquid inlet pipe 24, one end of the liquid inlet pipe 24 extends into the position near the bottom of the elution bottle 1, and the other end of the liquid inlet pipe 24 is connected with the liquid inlet 42 of the liquid separation valve 4, The liquid distribution valve 4 is also provided with a first liquid distribution port 43 and a second liquid distribution port 44 , and the first liquid distribution port 43 and the seco...

Embodiment 2

[0045] In a preferred scheme, the eluent is equipped with eluent in the elution bottle 1, and the eluent includes the minimum common component of the preservation solution and the lysate, the balance is water, and the minimum common component is the overlap between the preservation solution and the lysis solution. Components, take the minimum value in the preservation solution and the lysis solution;

[0046] Subtract the corresponding minimum common components from the preservation solution and the lysis solution. In this example, the scheme of eluting first and then separating liquid was adopted to solve the problem of sample uniformity. For example:

[0047] The components of the eluent are sodium chloride, with a mass percentage of 0.420%; concentrated hydrochloric acid, with a volume percentage of 0.062%; the balance is water; this scheme can improve the elution efficiency. And can simulate the environment inside the human body.

[0048] The preservation test tube 6 is...

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Abstract

The invention provides a liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus. The liquid separation device comprises a sealing cover, and the sealing cover is used for being hermetically connected with a bottle opening of an elution bottle; an air inlet pipe is arranged on the sealing cover, one end of the air inlet pipe communicates with an inner cavity of the elution bottle, and the other end of the air inlet pipe is connected with an air bag; and a liquid inlet pipe is further arranged on the sealing cover, one end of the liquid inlet pipe extends into the position, close to the bottom, in the elution bottle, the other end of the liquid inlet pipe is connected with a liquid inlet of a liquid separation valve, the liquid separation valve is further provided with a first liquid separation opening and a second liquid separation opening, and the first liquid separation opening and the second liquid separation opening communicate with the liquid inlet. According to the liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus, a sample can be correspondingly and independently mixed with a preservation solution and a lysis solution by one-time collection, so that the uniformity and independence of the sample are ensured, and the situation that the final detection result is influenced due to the fact that the samples collected twice are different is avoided, so that the accuracy of TCT and cervical HPV-DNA combined detection is improved.

Description

technical field [0001] The invention relates to the field of joint screening of cervical cancer, in particular to a liquid dispensing device for joint screening of cervical cancer by liquid-based cytology and human papillomavirus. Background technique [0002] In recent years, cervical cancer has shown a younger trend and is a common gynecological malignancy that seriously threatens women's health. The etiology of cervical cancer is clear, and high-risk human papillomavirus (HPV) infection is the primary condition leading to cervical cancer. Cervical cancer can be found, diagnosed and treated early, so early screening is an important measure to prevent and treat cervical cancer. With the development of cervical cancer screening technology in recent years, liquid-based thin-layer cytology screening (TCT), as an important diagnostic technique for early screening of cervical cancer lesions, plays a gold standard role in the diagnosis of cervical precancerous lesions, while HPV...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6806C12R1/93
CPCC12Q1/708C12Q1/6806
Inventor 郝宗杰庞宝川孙小蓉曹得华董明国罗强
Owner 武汉兰丁云医学检验实验室有限公司
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