Glass slide adhesive

A technology for adhesives and glass slides, which is applied in the field of anti-detachment treatment reagents for glass slides, which can solve the problems of poor adhesion and weak wettability, and achieve the effects of improved wettability, firm binding, and low energy consumption

Active Publication Date: 2015-03-18
MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a new type of adhesive for slides that can firmly adhere cells and tissue sections to slides and prevent them from falling off due to many factors such as overacid, high temperature, and high pressure during the staining process. In practical work, common adhesives often encounter the disadvantages of poor adhesion and weak wettability when used for slide glass processing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh or measure each component according to the table below, and prepare a 100ml solution.

[0021] 3-Aminopropyltriethoxysilane (APTES) 0.010ml

[0022] Coconut Oil Fatty Acid Diethanolamide 0.200ml

[0023] Polyethylene glycol 200 0.010ml

[0024] Polyethylene glycol 4000 (density 1.212g / ml) 0.020g

[0025] Isopropanol 1.000ml

[0026] Ultrapure water make up 100ml

[0027] After mixing the components, stir to dissolve, soak the glass slide for 5-10 minutes, and let it dry.

[0028] 400 slides were processed for cytology and histology, and conventional staining and Feulgen staining were used. The results showed that the cell shedding rate of various preparation and staining methods was 0%; the wettability of liquid-based cytology preparation was poor; the cleanliness of the slide surface was poor after treatment.

Embodiment 2

[0030] Weigh or measure each component according to the table below, and prepare a 100ml solution.

[0031] 3-Aminopropyltriethoxysilane (APTES) 0.100ml

[0032] Polyvinylpyrrolidone (PVP) (density 1.23-1.29g / ml) 0.200g

[0033] Polyethylene glycol 200 0.005ml

[0034] Ethanol 0.500ml

[0035] Glycerol 1.650ml

[0036] Ultrapure water make up 100ml

[0037] After mixing the components, stir to dissolve, soak the glass slide for 5-10 minutes, and let it dry.

[0038] 400 slides were processed for cytology and histology, and conventional staining and Feulgen staining were used. The results showed that the cell shedding rate of various preparation and staining methods was 0%; the liquid-based cytology preparation had poor wettability; the surface cleanliness of the treated slide was good.

Embodiment 3

[0040] Weigh or measure each component according to the table below, and prepare a 100ml solution.

[0041] 3-Aminopropyltriethoxysilane (APTES) 0.020ml

[0042] Polyvinylpyrrolidone (PVP) (density 1.23-1.29g / ml) 0.300g

[0043] Polyethylene glycol 4000 (density 1.212g / ml) 0.010g

[0044] Isopropanol 2.000ml

[0045] Ultrapure water make up 100ml

[0046] After mixing the components, stir to dissolve, soak the glass slide for 5-10 minutes, and let it dry.

[0047] 400 slides were processed for cytology and histology, and conventional staining and Feulgen staining were used. The results showed that the cell shedding rate of various preparation and staining methods was 0%; the preparation of liquid-based cytology had strong wettability; the cleanliness of the surface of the slide was good after treatment.

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PUM

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Abstract

The invention discloses a glass slide adhesive. The glass slide adhesive includes 0.01-0.2% by volume of 3-aminopropyltriethoxysilane (APTES), 0.01-3% by volume of a hydrophilic reagent, 0.005-0.05% by volume of a stabilizer, 0.5-5% by volume of alcohol, and the balance ultrapure water. The glass slide adhesive makes cells and tissue slices be firmly adhered on a glass slide, prevents the shedding of the cells and the tissue slices from the glass slide because of peracidity, high temperature, high pressure and the like in a dyeing process, overcomes the disadvantages of bad adhesiveness and weak wettability of general adhesives when the general adhesives are used for the glass slide processing in practical work, is suitable for the tissue slices, routine cell smears, fluid-based cytologic preparation, and manual drops, and has the advantages of good glass slide adherence effect, long preservation time, low cost, and high popularization values.

Description

technical field [0001] The invention relates to a glass slide anti-detachment treatment reagent for firmly affixing cells and tissue sections on a glass slide and preventing slides from dropping due to overacid, high pressure and many other factors during the staining process. Background technique [0002] Pathological techniques are changing with each passing day, especially immunohistochemistry and liquid-based cytology, which have developed rapidly in recent years. During the staining process, cells and tissue sections are stained by various methods, especially when encountering complex staining such as special staining, Feulgen staining, and immunohistochemistry, and are often subjected to factors such as high temperature, high pressure, radiation, and acid-base corrosion. Affected, tissue cells are easily detached from the slide. Slides treated with poor adhesives may also suffer from slide background staining, contamination, etc. It not only affects the daily work of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C03C23/00
Inventor 黄荣祥唐剑朱晨雁
Owner MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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