Cleaning fluid for pathologic cell sheet preparation technology and using method of cleaning fluid

A tableting technology and pathological cell technology, applied in the field of cleaning solution for pathological tableting, can solve the problems of restricting the popularization and application of technology, and achieve the effects of saving the time of reading the film, improving the adhesion, and reducing the misdiagnosis rate.

Inactive Publication Date: 2016-04-20
MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existing technical problems have seriously restricted the popularization and application of the above-mentioned technologies.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh or measure each component according to the table below, and prepare a 100g solution;

[0030] Carrageenan 0.100g

[0031] Ethanol 50g

[0032] Polysorbate 1.000g

[0033] 100g of ultrapure water

[0034] Stir and dissolve each component after mixing, set aside;

[0035] Prepare 200 cell sheets with liquid base, wash the above cleaning solution on the liquid-based cell sheet three times, 1ml each time; after the third rinse, let it stand for 1min, then discard the residual liquid of the cleaning solution; wash the liquid-based cell sheet with the cleaning solution Half of the slices were fixed in 95% ethanol, and the other half were dried directly. Feulgen staining and other methods were used to treat the cells. The results showed that the cell detachment rate of each treatment method was 0%; the background of the slides under the microscope was clean and the cells were less overlapping.

Embodiment 2

[0037] Weigh or measure each component according to the table below, and prepare a 100g solution.

[0038] Gum Arabic 0.160g

[0039] Ethanol 20g

[0040] Methanol 10g

[0041] Sodium dodecylbenzenesulfonate 0.100g

[0042] 100g of ultrapure water

[0043] Stir and dissolve each component after mixing, set aside;

[0044] Prepare 200 cell sheets with liquid base, wash the above cleaning solution on the liquid-based cell sheet three times, 1ml each time; after the third rinse, let it stand for 1min, then discard the residual liquid of the cleaning solution; wash the liquid-based cell sheet with the cleaning solution Half of the slices were fixed in 95% ethanol, and the other half were dried directly. Feulgen staining and other methods were used to treat the cells. The results showed that the cell detachment rate of each treatment method was 0%; the background of the slides under the microscope was clean and the cells were less overlapping.

Embodiment 3

[0046] Weigh or measure each component according to the table below, and prepare a 100g solution.

[0047] Gelatin 0.100g

[0048] Ethanol 40g

[0049] Polysorbate 0.100g

[0050] 100g of ultrapure water

[0051] Stir and dissolve each component after mixing, set aside;

[0052] Prepare 200 cell sheets with liquid base, wash the above cleaning solution on the liquid-based cell sheet three times, 1ml each time; after the third rinse, let it stand for 1min, then discard the residual liquid of the cleaning solution; wash the liquid-based cell sheet with the cleaning solution Half of the slices were fixed in 95% ethanol, and the other half were dried directly. Feulgen staining and other methods were used to treat the cells. The results showed that the cell detachment rate of each treatment method was 0%; the background of the slides under the microscope was clean and the cells were less overlapping.

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PUM

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Abstract

The invention discloses a cleaning fluid for a pathologic cell sheet preparation technology. The cleaning fluid is characterized by comprising the following components in percentage by mass: 0.01 to 0.50 percent of adhesive, 10 to 60 percent of a fixing agent, 0.10 to 1.5 percent of an activator, and the balance of ultrapure water. The invention further discloses a using method of the cleaning fluid for the pathologic cell sheet preparation technology. The cleaning fluid disclosed by the invention is used for treating liquid-based cell sheets, and has the advantages that the microscopically background cleanliness is extremely high, and cells cannot fall and the like; the treated cell sheets can achieve the effects of uniformity, consistency, clear color, clean background, no non-specific staining, and zero sheet falling rate.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a cleaning solution for pathological cell sheet preparation and a method for using the same, which can clean pathological cell sheets and prevent the sheet from being stripped due to staining. Background technique [0002] Pathological cell preparation technology is an integral part of pathological diagnosis, and the quality of preparation technology directly affects whether pathologists can make correct pathological diagnosis. [0003] The third-generation cytology production technology—liquid-based sedimentation production, entered the Chinese market at the end of 2001. Compared with the traditional Pap smear examination, it significantly improved the satisfaction of specimens and the detection rate of abnormal cells, and solved the problem. Traditional smears have high false negative rate, high rate of lost cells (80%), poor smear quality, excessive mucus, blood or inflammato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D1/74C11D3/20C11D3/37C11D3/60A61L2/18
CPCC11D1/74A61L2/0088C11D3/201C11D3/22
Inventor 黄荣祥朱晨雁
Owner MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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