A kind of anti-heparin stable α-l-fucosidase detection kit and its application
A technology of fucosidase and fucoside, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of non-resistance to heparin, and achieve the effects of convenient operation, removal of heparin interference, and short detection time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0029] The present invention preferably also provides a preparation method of the above-mentioned kit, comprising: mixing buffer, substrate, surfactant, streptomycin sulfate, stabilizer and preservative, adjusting the pH to 4.5-5.5, and distilling the volume to 1 L, Reagent R is obtained; the reagent R is subpackaged to obtain the above kit. In the present invention, the reagent for adjusting pH preferably includes hydrochloric acid or sodium hydroxide solution. In the present invention, there is no special requirement for the operation of adjusting the pH, and operations well known to those skilled in the art can be used.
[0030] The present invention also provides the application of the above kit in detecting α-L-fucosidase. In the present invention, the detected samples preferably include samples containing heparin. The detection of α-L-fucosidase by the kit of the present invention is for non-diagnostic purposes, and only for obtaining an intermediate value of α-L-fucos...
Embodiment 1
[0035] An anti-heparin stable α-L-fucosidase detection kit, the preparation of the kit: 300mmol acetic acid-sodium acetate buffer solution, 1g M-G-2-chloro-p-nitrophenol-α-L- Fucoside, 30g of Triton 100, 0.03g of streptomycin sulfate, 0.1g of L-cysteine and 2g of Proclin 300 were sequentially added into the beaker and stirred until completely dissolved. After adjusting the pH to 5.2 with hydrochloric acid and sodium hydroxide solution, Dilute to 1L to obtain reagent R, and divide reagent R into 60mL / bottles to obtain kits.
Embodiment 2
[0037] An anti-heparin stable α-L-fucosidase detection kit, the preparation of the kit: 300mmol acetic acid-sodium acetate buffer solution, 1g M-G-2-chloro-p-nitrophenol-α-L- Add fucoside, 30g Triton 100, 0.05g streptomycin sulfate, 0.05g L-cysteine and 2g Proclin 300 into the beaker and stir until completely dissolved, adjust the pH to 5.2 with hydrochloric acid and sodium hydroxide solution, Dilute to 1L to obtain reagent R, and divide reagent R into 60mL / bottles to obtain kits.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com