Cell extraction solution and application thereof
A technology for extracting liquid and cells, applied in the field of pharmacy, which can solve problems such as interference in doctor reading and diagnosis
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Embodiment 1
[0047] A cell extract, composed of the following mass fractions: glycerol 30%, potassium chloride 0.01%, potassium dihydrogen phosphate 0.02%, disodium hydrogen phosphate 0.15%, sodium chloride 0.8%, ethylenediaminetetraacetic acid di Sodium 0.6%, phenoxyethanol 0.1%, the remaining components are ultrapure water, and the specific gravity of the cell extract is 1.06.
Embodiment 2
[0049] A cell extract, composed of the following mass fractions: glycerol 35%, potassium chloride 0.01%, potassium dihydrogen phosphate 0.02%, disodium hydrogen phosphate 0.15%, sodium chloride 0.8%, ethylenediaminetetraacetic acid di Sodium 0.6%, phenoxyethanol 0.1%, the remaining components are ultrapure water, and the specific gravity of the cell extract is 1.07.
Embodiment 3
[0051] Sample experiment using the cell extract prepared in Example 1
[0052] 1. Preparation of relevant reagents:
[0053] 1.1 Buffer: prepare 10mM Tris buffer with pH 7.2.
[0054] 1.2 Rinsing solution: Mix absolute ethanol and isopropanol according to equal volumes.
[0055] 1.3 Papanicolaou staining solution: the Papanicolaou staining solution used in this example is the Papanicolaou staining solution produced by Xiaogan Hongxiang Biomedical Technology Co., Ltd.
[0056] 1.4 Liquid-based cell preservation solution sampling bottle: The liquid-based cell preservation solution used in this example is the liquid-based cell preservation solution produced by Xiaogan Hongxiang Biomedical Equipment Technology Co., Ltd.
[0057]2. Sample centrifugation and stratification: take an appropriate amount of centrifuge tubes and number them corresponding to the sample bottle of the liquid-based cell preservation solution, add 4 mL of the cell extract prepared in Example 1, and then tak...
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