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Manual semi-quantitative liquid-base cytological flaking method

A semi-quantitative and cytological technology, applied in the field of cytology film production, can solve the problems of high price of domestic film production system, high cost of supporting reagent consumables, unstable film production quality, etc., to improve the efficiency of film reading and easy to quickly master , strong repeatability and flexibility

Inactive Publication Date: 2012-10-24
MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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Problems solved by technology

[0004] The purpose of the present invention is to provide a manual semi-quantitative liquid-based cytology method, which is a low-cost, high-efficiency, repeatable, and controllable cervical liquid-based cytology technology to solve the problem of imported automatic film production. The equipment is expensive, the cost of supporting reagents and consumables is too high, the price of the domestic production system is high, the quality of the production is unstable, and the traditional manual drop method is rough.

Method used

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  • Manual semi-quantitative liquid-base cytological flaking method

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Embodiment Construction

[0025] The steps of the specific embodiment of the present invention are as follows:

[0026] 1. Specimen collection:

[0027] Use a cervical sampler to collect exfoliated cells from the cervix, remove the brush head of the cervical sampler and place it in the cell preservation solution in the specimen bottle.

[0028] 2. Cytology production:

[0029] (1) Specimen confirmation and numbering:

[0030] Take the sample tube to be tested and the application form, mark the serial number on the sample tube and the application form with a marker pen, and prepare 1 standard conical bottom centrifuge tube (12ml) and 1 centrifuge tube corresponding to the sample tube in the test tube rack. Round bottom test tubes (5ml), centrifuge tubes and round bottom test tubes are numbered with a marker pen, and this number should be consistent with the number of the sample tube.

[0031] (2) Add cell dispersant:

[0032] Add 100 μl of cell dispersant (Synermed, USA) to each round-bottom test tu...

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Abstract

The invention discloses a manual semi-quantitative liquid-base cytological flaking method. Cervical exfoliated cells stored with liquid phase are used as a specimen source, centrifugation and semi-quantitative dilution are carried out, a cell dispersing agent is added to carry out suspending and evenly mixing, cell suspension is taken and dropped on the surface of a glass slide, and fixing and dyeing are carried out after the cells are settled and dried naturally. The method comprises the following steps: confirming and numbering a specimen; adding the cell dispersing agent; transferring the specimen and carrying out centrifugation; pouring supernate; diluting cell precipitation; transferring the cell suspension; and slide-dropping, and finishing flaking. The cervical liquid-base cytological flaking method of the invention is low-cost, highly-efficient, repeatable, and controllable, which solves the current problems including that imported automatic flaking equipment is expensive and matching reagents and consumables are too high in cost, domestic flaking systems are expensive but flaking quality is not stable, and slides prepared by traditional manual slide-dropping methods are rough. Thus, the flaking method of the invention is suitable for popularization in multi-level hospitals and medical organizations, especially in primary-level organizations.

Description

technical field [0001] The invention relates to a method for preparing liquid-based cytology slices suitable for gynecological physical examination and cervical cancer screening, and belongs to the technical field of cytology slices. Background technique [0002] Liquid-based cytology examination is the use of liquid-based cytology film preparation and staining technology to detect cervical exfoliated cells and conduct cytological diagnosis. It is currently an advanced cervical cancer detection technology in the world. Compared with the smear examination, the satisfaction of the specimen and the detection rate of abnormal cervical cells are significantly improved. Liquid-based cytology technology is a technology that preserves the exfoliated cells in liquid, and evenly disperses and attaches the cells on the glass slide to make a smear through a special way. Liquid-based cytology production technology was born in the United States and other developed countries in 1991, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 唐剑
Owner MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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