Manual semi-quantitative liquid-base cytological flaking method

Abstract

The invention discloses a manual semi-quantitative liquid-base cytological flaking method. Cervical exfoliated cells stored with liquid phase are used as a specimen source, centrifugation and semi-quantitative dilution are carried out, a cell dispersing agent is added to carry out suspending and evenly mixing, cell suspension is taken and dropped on the surface of a glass slide, and fixing and dyeing are carried out after the cells are settled and dried naturally. The method comprises the following steps: confirming and numbering a specimen; adding the cell dispersing agent; transferring the specimen and carrying out centrifugation; pouring supernate; diluting cell precipitation; transferring the cell suspension; and slide-dropping, and finishing flaking. The cervical liquid-base cytological flaking method of the invention is low-cost, highly-efficient, repeatable, and controllable, which solves the current problems including that imported automatic flaking equipment is expensive and matching reagents and consumables are too high in cost, domestic flaking systems are expensive but flaking quality is not stable, and slides prepared by traditional manual slide-dropping methods are rough. Thus, the flaking method of the invention is suitable for popularization in multi-level hospitals and medical organizations, especially in primary-level organizations.

Description

technical field [0001] The invention relates to a method for preparing liquid-based cytology slices suitable for gynecological physical examination and cervical cancer screening, and belongs to the technical field of cytology slices. Background technique [0002] Liquid-based cytology examination is the use of liquid-based cytology film preparation and staining technology to detect cervical exfoliated cells and conduct cytological diagnosis. It is currently an advanced cervical cancer detection technology in the world. Compared with the smear examination, the satisfaction of the specimen and the detection rate of abnormal cervical cells are significantly improved. Liquid-based cytology technology is a technology that preserves the exfoliated cells in liquid, and evenly disperses and attaches the cells on the glass slide to make a smear through a special way. Liquid-based cytology production technology was born in the United States and other developed countries in 1991, and ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a manual semi-quantitative liquid-based cytology method, which is a low-cost, high-efficiency, repeatable, and controllable cervical liquid-based cytology technology to solve the problem of imported automatic film production. The equipment is expensive, the cost of supporting reagents and consumables is too high, the price of the domestic production system is high, the quality of the production is unstable, and the traditional manual drop method is rough.

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