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Antihypertensive peptide P16 as well as preparation method and application thereof

A blood pressure-lowering peptide, P16 technology, applied in the field of peptides, can solve problems such as unstandardized product quality, achieve huge practical value and economic benefits, good blood pressure lowering effect, and the effect of inhibiting the activity of ACE enzymes

Inactive Publication Date: 2014-09-24
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structural identification of the antihypertensive peptide component with the highest antihypertensive activity has not been carried out, and the product quality cannot be standardized

Method used

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  • Antihypertensive peptide P16 as well as preparation method and application thereof
  • Antihypertensive peptide P16 as well as preparation method and application thereof
  • Antihypertensive peptide P16 as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The amino acid sequence of the hypotensive peptide P16 provided in this example is shown in SEQ ID No.1.

[0031] The above-mentioned hypotensive peptide P16 is prepared by the following method:

[0032] (1) Add 4000mL of pure water to the beaker, stir at 200rpm, slowly add 1kg of casein (Fonterra food-grade imported casein, just listed, not limited to this) into the beaker, stir and dissolve at 40°C, Aqueous solution of casein;

[0033] (2) Adding trypsin to the aqueous solution of casein for enzymolysis, the enzymolysis conditions are: the amount of enzyme added is 0.1% of the total mass of casein, the reaction temperature is 40°C, the reaction time is 1h, and the pH is 7.0;

[0034] (3) Adjust the temperature to 80°C, keep it warm for 30 minutes, and sterilize;

[0035] (4) adjust the pH value to 4.0, and centrifuge at 3000r / min for 30min to remove unenzymolyzed macromolecular protein precipitates to obtain a supernatant containing highly active blood pressure-lowe...

Embodiment 2

[0058] The amino acid sequence of the hypotensive peptide P16 provided in this example is shown in SEQ ID No.1.

[0059] The above-mentioned high-purity hypotensive peptide P16 is prepared by the following method:

[0060] (1) Add 600mL of pure water to the beaker, stir at 200rpm, slowly put 100g of casein into the beaker, stir and dissolve at 50°C;

[0061] (2) Adding trypsin for enzymolysis, the enzymolysis conditions are: the amount of enzyme added is 0.2% of the casein mass, the reaction temperature is 50°C, the reaction time is 2h, and the pH is 9.0;

[0062] (3) Adjust the temperature to 90°C, keep it warm for 20 minutes, and inactivate the enzyme;

[0063] (4) adjust the pH value to 5.0, and centrifuge at 5000r / min for 20min to remove unenzymolyzed macromolecular protein precipitates;

[0064] (5) Reversed-phase silica gel purification, the eluate is then purified by reverse-phase silica gel, HPLC system: LC-8A Shimadzu preparative high-performance liquid chromatograp...

Embodiment 3

[0071] The amino acid sequence of the hypotensive peptide P16 provided in this example is shown in SEQ ID No.1.

[0072] The above-mentioned high-purity hypotensive peptide P16 is prepared by the following method:

[0073] (1) Take casein and dissolve it with water to obtain an aqueous solution of casein, wherein the amount of water is 5 times the total mass of casein, and the dissolution temperature is 50°C;

[0074] (2) Add trypsin to the aqueous solution of casein for enzymolysis, the amount of trypsin added accounts for 0.15% of the total mass of casein, the enzymolysis temperature is 45°C, the enzymolysis time is 1.5h, and the pH value is 8.0. Enzyme inactivation treatment was carried out after the hydrolysis, the temperature during inactivation was 85°C, and the inactivation time was 25min to obtain an enzyme inactivation solution;

[0075] (3) Adjusting the pH value to 4.5 so that the unenzymolyzed macromolecules in the enzyme-inactivating solution are precipitated for...

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Abstract

The invention discloses an antihypertensive peptide P16 which has the amino acid sequence shown as SEQ IDNo.1. The antihypertensive peptide P16 is clear and definite in structure and good in antihypertensive activity. The invention further discloses a preparation method of the antihypertensive peptide P16. The preparation method has a compact process and realizes the standardization of the quality of the antihypertensive peptide P16. The invention further discloses an application of the antihypertensive peptide P16 in preparation of a medicine or a food with an antihypertensive function.

Description

technical field [0001] The invention belongs to the technical field of polypeptides, and in particular relates to a blood pressure-lowering peptide P16 and its preparation method and application. Background technique [0002] Hypertension is a common cardiovascular disease. It not only has a high prevalence rate, but also often causes serious heart, brain, kidney and other diseases. It is a major risk factor for stroke, coronary heart disease, heart failure and kidney disease. Hypotensive peptides are a class of peptide substances that have the effect of hypotensive peptides. Although ACE inhibitory peptides obtained from food proteins are weaker than artificially synthesized ACEI, their importance lies in the potential of these relatively weak hypotensive substances in our Among the daily intake of food, it has its unique advantages: first, it can only lower blood pressure for hypertensive patients, and has no blood pressure lowering effect on normal blood pressure, so it w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/20C12P21/06A61K38/08A61P9/12A23L1/29A23L33/00
Inventor 宁德山赵力超马忠华曹庸郭晓蕾刘飞杜洁陈飞龙吴成顺
Owner INFINITUS (CHINA) CO LTD
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