Detection method of L-arginine and sensor

A detection method, technology of arginine, applied in the field of chemical/biological sensing

Pending Publication Date: 2019-09-10
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no enzyme-free electrochemical sensor for detecting L-arginine based on a polypeptide composite membrane has been reported so far.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of L-arginine and sensor
  • Detection method of L-arginine and sensor
  • Detection method of L-arginine and sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] In the embodiment, the polypeptide mother solution (1.0mmol / L) was prepared with ultrapure water, and diluted to the required concentration with phosphate buffered saline solution (PBS, 10mmol / L, pH=7.4) when used, 0.20mol / L of NaOH and 0.20mol / L HCl is used to adjust the pH value of the phosphate buffer solution; the reagents used are all analytical grade (AR), and the experimental water is ultrapure water (resistivity ≥ 18.3MΩ·cm). In the following description, the descriptions of amino acids all use English abbreviations.

[0028] 1. Experimental process

[0029] 1. Preparation of FC-P16 Peptide / AuE Polypeptide Composite Membrane Modified Electrode

[0030] The gold electrode (diameter 3mm) was soaked in Piranha solution for 5min and washed with ultrapure water. Use 1.0μm, 0.3μm, 0.05μm Al in turn 2 o 3 The powder was ground and polished into a mirror surface, ultrasonicated in ultrapure water, absolute ethanol, and ultrapure water for 5 minutes, cleaned and clea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a detection method of L-arginine (L-Arg) and a sensor. The method comprises the steps of: synthesizing ferrocene functional hexadeceptide dithio cyclopentane (FC-P16 Peptide);preparing a FC-P16 Peptide / AuE polypeptide composite membrane modified electrode; carrying out L-Arg detection; and the like. A result shows that the FC-P16 Peptide / AuE polypeptide composite membranemodified electrode shows the excellent electrochemical response characteristic for the L-Arg. In 10mmol / L PBS (Phosphate Buffered Solution) (pH is 7.4), a DPV response peak current of the polypeptidecomposite membrane modified electrode and concentration of the L-Arg has an excellent linear relationship within a range of 1.0*10<-13> to 1.0*10<-7> mol / L; and a detection limit is 1.0*10<-13> mol / L.The modified electrode has excellent reproducibility, repeatability and selectivity, and has potential application value in the aspects of life sciences and nutrition and health.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing, and in particular relates to a detection method and sensor of L-arginine. Background technique [0002] L-arginine (L-arginine, L-Arg), also known as protein amino acid, is an essential amino acid for maintaining the growth and development of infants. It is an intermediate metabolite of the ornithine cycle, which can promote the conversion of ammonia into urea, thereby reducing blood ammonia levels. It helps improve immune system health and fight disease. In the event of a physical injury, the body's immune system is at its best, allowing the body to heal faster. Overall, arginine plays an extremely important role in wound healing, cell division, maintaining the body's charge balance, and physiological functions. Therefore, developing a rapid and sensitive method for detecting L-Arg is of great significance in the fields of life science and nutrition and health. [0003] A...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/48G01N33/5438G01N33/6812C07K1/1077
Inventor 曹忠周宁涛周立朱钦何婧琳肖忠良贺玉敏冯泽猛印遇龙
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap