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Method for enriching target regions of 168 genes based on multi-probes

A target area, multi-probe technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of high cost

Inactive Publication Date: 2015-12-02
GUANGZHOU BURNING ROCK DX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Exome sequencing does not require any prior knowledge, but the detection of rare mutations requires deep sequencing, which is very costly

Method used

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  • Method for enriching target regions of 168 genes based on multi-probes
  • Method for enriching target regions of 168 genes based on multi-probes
  • Method for enriching target regions of 168 genes based on multi-probes

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Embodiment Construction

[0112] The present invention will be further described below in conjunction with the accompanying drawings. It should be noted that this embodiment is based on the technical solution, and provides detailed implementation and specific operation process, but the protection scope of the present invention is not limited to the present invention. Example.

[0113] Such as figure 1As shown, the implementation process of the present invention is specifically as follows:

[0114] Pre-Library Preparation (50ngDNAsamples)

[0115] 1. gDNA fragmentation and adapter adapter

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Abstract

The invention discloses a method for enriching target regions of 168 genes based on multi-probes. According to the invention, 168 genes originated from a TCGA database, targets of conventional approved targeted drugs for lung cancer, targets of targeted drugs for other tumors and targets of targeted drugs in clinical experiments are combined together, and multi-target sequences of the 168 genes are enriched in one shot by using probe capture technology; so multi-gene multi-target parallel in-depth high flux sequencing is realized, a plurality of variation types like mutation, deletion, insertion, fusion and copy number amplification of genes are examined at the same time, and the method is superior to the prior art in detection depth and width.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for enriching 168 gene target regions based on multiple probes. Background technique [0002] As cancer cells rupture and die, they shed contents such as circulating tumor DNA (ctDNA), fragments of the genome that float in the blood. Cellular debris is generally removed by scavenger cells (such as macrophages), but tumor cells are large and proliferate rapidly, making scavenger cells unable to cope. Detecting and sequencing tumor DNA in blood can help people get more comprehensive cancer information by taking blood. [0003] In 2012, Charles Swanton of Cancer Research UK found that when DNA sequencing of kidney tumors was performed, there was an astonishing genetic diversity in a single tumor, and only one-third of the mutations were shared by the whole. In addition, secondary tumors formed by cancer metastasis are also very different from the primary tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2535/122C12Q2525/191C12Q2563/149C12Q2563/143
Inventor 汉雨生揣少坤段飞蝶
Owner GUANGZHOU BURNING ROCK DX CO LTD