Breeding method and application of new recessive dwarf rape
A technology of brassica napus and dwarf stalks, which is applied in the fields of application, botany equipment and methods, plant gene improvement, etc., can solve the problems of lodging reduction, plant height, etc., and achieve the effect of increasing unit yield and solving plant height
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Embodiment 1
[0017] Embodiment 1 of the present invention: Breeding of a new recessive dwarf rapeseed:
[0018] 1) Hybridization test: The Brassica napus variety Jingyou No. 2 was used as the female parent and the Brassica napus variety Deyou No. 5 was used as the male parent to obtain F 1 , bagging self-breeding to obtain segregated populations;
[0019] 2) Acquisition of a new recessive dwarf rapeseed: on 410 F 2 The traits of plant height, number of primary branches, length of main inflorescence, number of siliques in main inflorescence, length of siliques and number of seeds per horn were identified for a single plant, and traits such as mustard, glucosinolate, oil content and protein content were detected at the same time, and 1 Dwarf stalked rapeseed; identified at multiple points (Guiyang Jinyang, Guizhou Tangtou) and for many years (2005-2011), the plant type, plant height, branches and main inflorescence are very stable. These traits are specifically shown as: plant height ...
Embodiment 2
[0020] Embodiment 2 of the present invention: Genetic identification of a novel recessive dwarf trait:
[0021] 1) Dominance and recessiveness of dwarf stem traits: Reciprocal cross between dwarf material and tall Brassica napus variety Zhongshuang 11, F 1 The plants all showed a phenotype similar to that of Zhongshuang 11, indicating that the short stem is recessive to the tall stem, and there is no cytoplasmic effect; the F of plant height and related traits 2 The traits of the population were segregated, showing continuous distribution characteristics, typical quantitative inheritance characteristics, and multimodal distribution at the same time. It was inferred that the dwarf trait may have a major gene effect;
[0022] 2) Genetic analysis and identification of new dwarf traits: F 1 (P 1 ×P 2 ,P 2 ×P 1 ), F 1 (P 2 ×P 1 ) selfing seeds to get F 2 , and cross to obtain B 1 (F 1 ×P 1 ) and B2(F 1 ×P 2 ), to construct a 6-generation population (P 1 ,P 2 ,...
Embodiment 3
[0023] Embodiment 3 of the present invention: Application of a new type of recessive dwarf rapeseed in breeding semi-dwarf rapeseed:
[0024] F 1 , a segregated population was obtained by selfing, with a total of 521 F 2 For a single plant, a semi-dwarf material with a plant height of about 1.5m can be obtained through continuous self-crossing and backcrossing.
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