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Method for widening the cell hereditable basic of cultivated soybeans using wild soybeans

A technology for cultivating soybeans and wild soybeans, applied in the fields of botany equipment and methods, application, plant genetic improvement, etc., to achieve the effect of stabilizing beneficial traits

Inactive Publication Date: 2005-12-28
CHENGDE YINGZE SEED SCI & TECH DEV
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Problems solved by technology

[0003] After retrieval by the Science and Technology Documentation Center of the Chinese Academy of Agricultural Sciences, China AGRIS North China Branch Center, and the Information Research Institute of the Hebei Provincial Academy of Agricultural Sciences, the research literature on wild soybeans was searched. Agriculture and Animal Husbandry Information Research", 1982, No. 20, p. 51) used wild soybean as the female parent to cross the cultivated soybean once, and observed the genetic segregation performance of the first to third generation traits. There is no wild soybean as the female parent at home and abroad This research report on the effect of hybridization and generalized backcrossing with cultivated soybeans (see "Agricultural Science and Technology Highlights" edited by the Chinese Academy of Agricultural Sciences, 1993, No. 18, page 5)

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  • Method for widening the cell hereditable basic of cultivated soybeans using wild soybeans

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Effect test

Embodiment 1

[0044] (1) Obtain F1 generation true hybrids with wild soybean as female parent

[0045] In 1984, high-protein Guxin wild soybeans were planted, and the flowering period was observed. The Guxin wild soybeans from southern Hebei were late-maturing in Chengde, northern Hebei, and flowering began on August 20. At this time, all spring soybeans All the varieties have finished blooming, and the work of pollination and hybridization has not been completed. It was sown again in 1985. After the seedlings emerged, they were treated with short-day light for 20 days. They were shaded from 5:00 pm to 8:00 the next day, so that they bloomed on June 23, 57 days earlier than under natural conditions, creating a good environment for hybridization. condition. In order to reduce the plant height and increase the high yield of interspecific hybrid offspring, a limited plant height of 60 cm and good high yield Chengdou No. 1 was selected as the male parent for hybridization, and one F0 generatio...

Embodiment 2

[0051] In order to prove the reproducibility of successful hybridization between wild soybeans and cultivated soybeans, Kongqiao wild soybeans were used as female parents in 1994. After 18 days of short-day treatment, after flowering, Henan soybeans with limited grain size were used as male parents. The hybridization was successful again, and the combination number was 9441. Both F1 and F2 were crossed in a broad sense, and the F3 substitute Zhongpin 661 was crossed in a broad sense, and 5 F0 seeds were obtained, and the combination number was 9718. In the future, use 9718F4 and 9441-7 as the female parent, and use multiple cultivated soybeans such as Yudou 18, Chengdou 5, Zhongpin 5016, Yudou 12, Wuyi Yanzhongying soybean, Jiqing No. 1, 9039-1-8= 1 et al. conducted generalized hybridization and now obtained a batch (204 of 10 combinations) of interspecific hybrid progeny materials with thick stems, large grains, and wild soybean cytoplasm. This batch of materials is being sele...

Embodiment 3

[0053] In order to broaden the cytoplasmic genetic basis of cultivated soybean and to reveal the reason and mechanism of the narrow and single cytoplasmic genetic basis of cultivated soybean in my country, in 1995, wild soybean Xinmin No. 6 was used as the parent, and four cultivated soybeans Hefeng No. 8412, Kai 81 57 Soybean conducted 4 pairs of reciprocal crossing experiments. From 1996 to 1998, the main agronomic traits segregation and maternal effect of F1, F2, F3 generation reciprocal crossing experiments were observed and analyzed in detail.

[0054] The test results showed that plant height had a very significant maternal effect (reached 0.01% significant). The number of main stem nodes and the number of pods per plant also had maternal effects. The number of grains per plant has a significant maternal effect, reaching 0.05% significant. The last pair of combinations of 100-kernel weight showed maternal effect. The first three combinations showed the dominant effect o...

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Abstract

The existing technical problem in soybean breeding is that the genetic base is getting narrower and narrower. Using wild soybeans as female parents can broaden the cytoplasmic genetic base of cultivated soybeans. In order to solve this technical problem, the technical scheme of the present invention is to use wild soybeans as the female parent, and use the first cultivated soybean as the male parent to carry out interspecific hybridization, and then use the different generation materials of the interspecific hybrid progeny with the second Hybrid breeding of cultivated soybeans. The technical effect of this technical solution is to broaden the genetic basis of cultivated soybean cytoplasm. According to the selected breeding goals, the varieties bred will increase yield by 11.1-27.2%, increase protein by 4.89-7.4%, have low insectivority rate, and have high or medium resistance. It is resistant to soybean mosaic virus disease No. 3, and has good agronomic properties.

Description

[technical field] [0001] The invention relates to the field of crop genetics and breeding, in particular to the technical field of wild soybean cytoplasm utilization in soybean germplasm innovation. [Background technique] [0002] At present, the genetic basis of soybean varieties is becoming narrower and narrower. In order to change this situation, breeders are trying to broaden the genetic basis of cultivated soybeans. Wild soybean has high protein content and strong stress resistance. It has become an important germplasm resource for broadening the genetic basis of cultivated soybean, and has attracted the attention and utilization of many breeders. However, it is generally believed that wild soybeans are not easy to overcome the shortcomings of creeping lodging, pod splitting, small grains, thin stems, and dark seed coats. At the same time, because the ovary and embryo capsule of wild soybean are very small, it is generally not easy to succeed in hybridization with cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 李玉清
Owner CHENGDE YINGZE SEED SCI & TECH DEV
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