Method utilizing protoplasts to fuse and screen rich-selenium high-yield strains among different ganoderma varieties

A protoplast fusion and protoplast technology, which is applied in the field of biological agriculture, can solve the problems such as no new selenium-enriched and high-yielding strains, etc., and achieve the effect of good economic benefits

Inactive Publication Date: 2012-02-15
无锡五芝源生物农业科技有限公司 +1
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Problems solved by technology

[0005] So far, there has not been any report on the screening of new selenium-enriched and high-yielding strains of different Ganoderma lucidum species through protoplast fusion

Method used

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  • Method utilizing protoplasts to fuse and screen rich-selenium high-yield strains among different ganoderma varieties
  • Method utilizing protoplasts to fuse and screen rich-selenium high-yield strains among different ganoderma varieties
  • Method utilizing protoplasts to fuse and screen rich-selenium high-yield strains among different ganoderma varieties

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Embodiment Construction

[0055] Below by example the present invention is described further: following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following example.

[0056] The method for screening new selenium-enriched and high-yield strains through protoplast fusion of different ganoderma varieties among ganoderma genus, the steps are:

[0057] 1. Screening of starting strains

[0058] 20 strains of different varieties of Ganoderma lucidum were introduced from the main production areas of Ganoderma lucidum in various provinces in China. Inoculated on PDA slant medium, and cultured at 28°C in the dark for 7 days for activation. The activated bacterial strain is inoculated in the center of the petri dish equipped with PDA medium, the germination time of Ganoderma lucidum mycelium is recorded, its growth rate is measured, and the morphology of mycelium (thickness and density of mycelium) is observed. Then carry out the Ganoderma lucidum liq...

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Abstract

A method utilizing protoplasts to fuse and screen rich-selenium high-yield strains among different ganoderma varieties belongs to the technical field of biological husbandry. The method selects different ganodermas to produce strains, the ganoderma cultivation is carried out, the ganoderma strains with high ganoderma sporocarp yield and good product agronomic trait are selected as the high-yield fusion original strains; and the selenium-resistant experiment is utilized to screen the ganoderma strains with strong rich-selenium capability as the rich-selenium fusion original strains. The method determines one parent strain as the inactivation strain through the hyphal growth speed and form, pigmental secretion, protoplast regeneration rate, protoplast inactivation time and the like; then the fusant screening is carried out through the hyphal form and antagonism, so the operation simplicity and practicability are obtained. The method is adopted to fuse one ganoderma fusion strain which has the sporocarp selenium content and yield higher than those of the original strain, so a good economical benefit is created.

Description

technical field [0001] The invention relates to a method for screening selenium-enriched high-yield strains through protoplast fusion among different ganoderma lucidum varieties, and belongs to the technical field of biological agriculture. Background technique [0002] Ganoderma lucidum is a fungus of the genus Ganoderma in the family Polyporaceae of Basidiomycetes, among which red lucidum is the most widely developed and used at present, and its fruiting body is one of the main raw materials for deep processing. Many studies have now confirmed that Ganoderma lucidum has the functions of regulating immunity, anti-oxidation, anti-hypoxia, anti-radiation, anti-chemotherapy, anti-myocardial ischemia and anti-aging effects, as well as sedation, cardiac strengthening, blood lipid regulation, hypoglycemia, asthma, and protection. Liver and other functions, widely used in the clinical treatment of various diseases. Ganoderma lucidum strains with high yield and excellent biologica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/04C12N1/14
Inventor 何静霞汪洁娄亚威刘广建全卫丰季宏更薛璟
Owner 无锡五芝源生物农业科技有限公司
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