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Enzymolytic preparation method for glossy ganoderma amylose

A technology of Ganoderma lucidum polysaccharide and enzymolysis, which is applied in the production field of active Ganoderma lucidum polysaccharide content

Inactive Publication Date: 2005-01-26
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the research on improving the content of active ganoderma lucidum polysaccharides by enzymatic methods, especially by the mycelia's own enzyme system and external enzyme system to treat the fermentation broth (containing mycelia).

Method used

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  • Enzymolytic preparation method for glossy ganoderma amylose
  • Enzymolytic preparation method for glossy ganoderma amylose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 10 tons of fermented liquid Ganoderma lucidum polysaccharide enzyme treatment method

[0026] (1) Obtain Ganoderma lucidum liquid submerged fermentation broth by step-by-step expansion of slant strains: connect the ganoderma lucidum slant strains into 500 ml shake flasks with 100 ml medium for primary seed cultivation, culture conditions: rotary shaker 150 rpm, 28°C for 50 hours. Then the seeds of the primary shake flask were inserted into a 500 ml shake flask equipped with 100 ml of medium with 10% inoculum to carry out secondary seed culture, and cultivated according to the same conditions as the primary shake flask. Then with 10% inoculum size, the secondary seeds are transferred into the 5000 milliliter shake flasks with 1000 milliliters of medium to carry out the three-stage seed cultivation, culture conditions: 180 rpm of rotary shaker, cultivated for 50 hours at 28°C . Then with 10% inoculum size, the third-level seeds are inserted into the first-level seed tan...

Embodiment 2

[0046] Embodiment 2 (10 tons of fermented liquid)

[0047] A kind of method that enzymolysis prepares Ganoderma lucidum polysaccharide, its step is the same as embodiment 1:

[0048] During the enzymatic hydrolysis process of the mycelium itself, the fermentation broth is adjusted to make its pH 5.5. At 45°C, the Ganoderma lucidum mycelium in the fermentation broth is enzymolyzed with the help of its own enzyme system existing in the Ganoderma lucidum mycelium. Solution for 70 minutes, stirring speed 40 rpm;

[0049] In the enzymatic hydrolysis process with external enzymes, at 50°C and pH 5.5, add mixed enzymes to the fermentation broth: 0.15 kg of cellulase, 1.2 kg of β-glucanase, 0.9 kg of pectinase, neutral Protease 0.75 kg, enzymolysis time 2 hours, stirring speed 40 rpm;

[0050] During the enzyme inactivation process, the fermentation broth was heated to 90°C for 10 minutes to inactivate the enzyme;

[0051] During the concentration process, the filtrate was concentr...

Embodiment 3

[0053] Embodiment 3 (10 tons of fermented liquid)

[0054] A kind of method that enzymolysis prepares Ganoderma lucidum polysaccharide, its step is the same as embodiment 1:

[0055] During the enzymatic hydrolysis process of the mycelium itself, adjust the fermentation broth to make it pH 5, and at 30°C, enzymolyze the Ganoderma lucidum mycelium in the fermentation broth with the help of the self-enzyme system existing in the ganoderma lucidum mycelium. Solution for 30 minutes, stirring speed 60 rpm;

[0056] In the enzymatic hydrolysis process with external enzymes, at 30°C and pH 6.0, add mixed enzymes to the fermentation broth: 0.2 kg of cellulase, 1.6 kg of β-glucanase, 1.2 kg of pectinase, neutral Protease 1 kg, enzymolysis time 2.5 hours, stirring speed 50 rpm;

[0057] In the process of inactivating the enzyme, the fermented liquid is heated to 100°C for 30 minutes to inactivate the enzyme;

[0058] During the concentration process, the filtrate was concentrated to ...

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Abstract

The invention discloses an enzymolytic preparation method for glossy ganoderma amylose which comprises the steps of, employing inclined-plane bacterial gradual cultivation to obtain the glossy ganoderma fungi fermentation liquid, adjusting fermentation liquid pH and temperature, carrying out enzyme decomposition to the glossy ganoderma mycelia by means of self enzyme group existed in glossy ganoderma mycelia, charging into the fermentation liquor by cellulase, beta-dextranase, pectin enzyme and neutral proteinase, carrying out hydrolytic decomposition, enzyme eradication, filtering, concentrating, alcohol depositing and separating.

Description

technical field [0001] The invention belongs to the field of fungal fermentation industry, and in particular relates to a production method for enzymatically treating ganoderma submerged fermentation broth to increase the content of active ganoderma polysaccharides. Background technique [0002] Ganoderma lucidum (Leyss ex Fr.) karst], also known as Ganoderma lucidum, belongs to Basidiomycotina, Polyporacea, Ganodermaceae, and Ganoderma genus. The medical codes of the past dynasties classified Ganoderma lucidum as the "shangshang medicine", which ranked before ginseng. "Shen Nong's Materia Medica" and "Compendium of Materia Medica" record that Ganoderma lucidum can improve eyesight, nourish liver qi, soothe the nerves, benefit essence, benefit heart qi, benefit spleen, benefit lung qi, benefit kidney qi, open nine orifices, deaf ears, eyesight, benefit Joints, diuresis, beauty and beauty, is a good nourishing product. [0003] Ganoderma lucidum polysaccharide can promote t...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P19/04
Inventor 蔡永峰程池黄宇彤岳国海李勣
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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