Culture method and applications of Ganoderma weberianum mycelial pellets

A technology of ganoderma lucidum mycelium and cultivation method, which is applied in the direction of chemical instruments and methods, fungi, water/sludge/sewage treatment, etc. It can solve the problems of low chromaticity removal efficiency and long reaction time, and achieve convenient operation Extensive, low cost, high decolorization degradation activity effect

Active Publication Date: 2011-05-25
广东一家亲营养科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The currently used denim printing and dyeing wastewater treatment methods are mainly traditional physical, chemical and biological (mainly

Method used

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  • Culture method and applications of Ganoderma weberianum mycelial pellets
  • Culture method and applications of Ganoderma weberianum mycelial pellets
  • Culture method and applications of Ganoderma weberianum mycelial pellets

Examples

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preparation example Construction

[0038] 1. Preparation of Weber Ganoderma lucidum mycelium balls

[0039] Under sterile conditions, use an inoculation loop to dig out the mycelia from the slant strains and transfer them to the plate medium, and put them in an incubator at 25-32°C for 2-5 days to obtain activated strains such as figure 1 with figure 2 shown. Then under aseptic conditions, dig mycelium from the plate of activated strains, disperse evenly, add it to the liquid culture medium containing Weber Ganoderma lucidum mycelium balls, under the conditions of 25-32°C and 120-200 rpm Vibrating culture for 3-6 days can obtain Weber ganoderma mycelium balls. The optimum condition for the activation of strains is to culture in an incubator at 28°C for 4 days; the optimum condition for obtaining Weber's Ganoderma lucidum mycelium ball is to culture with shaking at 28°C and 160 rpm for 5 days. image 3 Shown are mycelial pellets of various sizes obtained using this method.

[0040] The medium formula used i...

Embodiment 1

[0050] Add indigo solution to the culture of Weber ganoderma lucidum hyphae bulbs obtained by the method of the present invention to make the concentration 20mg / L, and adjust the acidity and alkalinity of the solution to pH4, and cultivate it statically for 2 hours at 28°C, and the indigo The decolorization rate is 41% as Figure 4 shown.

Embodiment 2

[0052] Add indigo solution to the Weber ganoderma mycelium culture obtained by the method of the present invention to make the concentration 20mg / L, and adjust the acidity and alkalinity of the solution to pH4, shake at 28°C and 160 rpm After culturing for 2 hours, the decolorization rate of indigo is 88%. Figure 4 shown.

[0053] Comparing Examples 1 and 2, it can be seen that shaking culture is beneficial to decolorization, and shaking culture is used in the following examples.

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Abstract

The invention provides a culture method and applications of Ganoderma weberianum mycelial pellets, belonging to the biological technical field. The culture method comprises the following steps: preculturing on a shelving medium slant to activate strains, transferring the activated strains to plating medium to culture and obtain plating strains; and transferring the plating strains to liquid medium to perform shaking culture under controlled conditions and obtain the mycelial pellets. The mycelial pellet culture prepared by the method is mixed with 0.5-10 times of indigo solution or denim printing and dying wastewater by volume to perform shaking culture for decoloration and degradation under the conditions that the pH value is 3-7, the temperature is 13-38 DEG C and the rotational speed is 160rpm or the conditions that the pH value is 2-6, the temperature is 23-43 and the rotational speed is 160rpm. The invention has the following advantages: the cell immobilization cost is saved, industrial production is easy to realize; decoloration and degradation can be performed without using toxic and expensive medium, thus the method is green and environmentally friendly; and the service life of the mycelial pellets is long, the mycelial pellets can be used repeatedly; and the method is convenient and extensive to operate and the cost is low.

Description

technical field [0001] The invention relates to a method for cultivating Weber ganoderma mycelium balls and its application, and belongs to the field of biotechnology. Specifically, the Weber ganoderma cells are automatically agglomerated and flocculated to form mycelium balls without adding an immobilization medium. The obtained mycelium balls are then used to decolorize and degrade the indigo and its printing and dyeing wastewater. Background technique [0002] In 2006, the total discharge of wastewater in my country was 53.68 billion tons, including 24.02 billion tons of industrial wastewater and 1.98 billion tons of printing and dyeing wastewater. 6th in the industrial sector. Therefore, the printing and dyeing industry is one of the key industrial polluting industries in my country. At present, more than 10,000 kinds of dyes are widely used in the production process of textile printing and dyeing, resulting in a large amount of colored wastewater. Because dyes are des...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C02F103/30
Inventor 田长恩周玉萍陈琼华
Owner 广东一家亲营养科技有限公司
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