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Method for increasing content of intracellular ganoderma lucidum triterpenes during liquid fermentation of ganoderma lucidum mycelia

A technology of Ganoderma lucidum mycelium and liquid fermentation, applied in the biological field, can solve problems such as bad residues, and achieve the effect of increasing content and safe price

Active Publication Date: 2016-04-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above defects or improvement needs of the prior art, the present invention provides a method for increasing the content of ganoderma triterpenoids in the liquid fermentation of ganoderma mycelia, the purpose of which is to induce ganoderma mycelia to synthesize ganoderma through natural wood degradation products Triterpenes, thereby increasing its yield, thereby solving the technical problem that the existing technology may have undesirable residues in increasing the content of Ganoderma lucidum triterpenes

Method used

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  • Method for increasing content of intracellular ganoderma lucidum triterpenes during liquid fermentation of ganoderma lucidum mycelia
  • Method for increasing content of intracellular ganoderma lucidum triterpenes during liquid fermentation of ganoderma lucidum mycelia
  • Method for increasing content of intracellular ganoderma lucidum triterpenes during liquid fermentation of ganoderma lucidum mycelia

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Embodiment 1

[0040] A method for improving the content of intracellular ganoderma triterpenoids in liquid fermentation of ganoderma mycelia, comprising the following steps:

[0041] (1) Activation of seeds: the ganoderma strains were activated with PDA liquid medium at a temperature of 28-30° C. and a rotation speed of 120 to 150 r / min for 7 days to obtain the ganoderma seeds. The PDA liquid medium contains per liter: 1 L of potato extract, 20 g of glucose, and 15 g of agar.

[0042] (2) Primary seed cultivation: Inoculate the mycelia of Ganoderma lucidum into the medium of Ganoderma lucidum at a volume ratio of 1:5, and cultivate for 5 days at a temperature of 28-30°C and a rotational speed of 120 to 150r / min to obtain a primary seed liquid. The ganoderma lucidum seed medium contains: glucose 35g / L, peptone 5g / L, yeast powder 2.5g / L, potassium dihydrogen phosphate 0.883g / L, magnesium sulfate heptahydrate 0.5g / L, and vitamin B10.05g / L .

[0043](3) Secondary seed cultivation, inoculate p...

Embodiment 2

[0050] A method for improving the content of Ganoderma lucidum triterpenoids in the bag of Ganoderma lucidum mycelia liquid fermentation, comprising the following steps:

[0051] (1) Activation of seeds: the ganoderma strains were activated with PDA liquid medium at a temperature of 28-30° C. and a rotation speed of 120 to 150 r / min for 7 days to obtain the ganoderma seeds. The PDA liquid medium contains per liter: 1 L of potato extract, 20 g of glucose, and 15 g of agar.

[0052] (2) Primary seed cultivation: Inoculate the mycelia of Ganoderma lucidum into the medium of Ganoderma lucidum at a volume ratio of 1:5, and cultivate for 5 days at a temperature of 28-30°C and a rotational speed of 120 to 150r / min to obtain a primary seed liquid. The ganoderma lucidum seed medium contains: glucose 35g / L, peptone 5g / L, yeast powder 2.5g / L, potassium dihydrogen phosphate 0.883g / L, magnesium sulfate heptahydrate 0.5g / L, and vitamin B10.05g / L .

[0053] (3) Secondary seed cultivation, ...

Embodiment 3

[0060] A method for improving the content of Ganoderma lucidum triterpenoids in the bag of Ganoderma lucidum mycelia liquid fermentation, comprising the following steps:

[0061] (1) Activation of seeds: the ganoderma strains were activated with PDA liquid medium at a temperature of 28-30° C. and a rotation speed of 120 to 150 r / min for 7 days to obtain the ganoderma seeds. The PDA liquid medium contains per liter: 1 L of potato extract, 20 g of glucose, and 15 g of agar.

[0062] (2) Primary seed cultivation: Inoculate the mycelia of Ganoderma lucidum into the medium of Ganoderma lucidum at a volume ratio of 1:5, and cultivate for 5 days at a temperature of 28-30°C and a rotational speed of 120 to 150r / min to obtain a primary seed liquid. The ganoderma lucidum seed medium contains: glucose 35g / L, peptone 5g / L, yeast powder 2.5g / L, potassium dihydrogen phosphate 0.883g / L, magnesium sulfate heptahydrate 0.5g / L, and vitamin B10.05g / L .

[0063] (3) Secondary seed cultivation, ...

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Abstract

The invention discloses a method for increasing the content of intracellular ganoderma lucidum triterpenes during liquid fermentation of ganoderma lucidum mycelia. The method is characterized by comprising the steps of performing enlarged cultivation, adding a wood degradation product into a culture solution according to a mass proportion of 0.01 to 5 percent, and performing culture for 1 to 6 days. According to the method for increasing the content of the intracellular ganoderma lucidum triterpenes during liquid fermentation of the ganoderma lucidum mycelia, microcrystalline cellulose is added to induce ganoderma lucidum to produce more intracellular ganoderma lucidum triterpenes during liquid fermentation of the ganoderma lucidum mycelia; the adopted inducer microcrystalline cellulose is safe and low in cost, and the content of the intracellular ganoderma lucidum triterpenes can be remarkably increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for increasing the content of intracellular ganoderma triterpenoids in liquid fermentation of ganoderma mycelia. Background technique [0002] Ganoderma lucidum (Ganodermalucidum) is a large medicinal fungus belonging to Basidiomycetes, Polyporaceae, and Ganoderma genus. It has been used in my country for thousands of years. Modern science has confirmed that the chemical components of Ganoderma lucidum include polysaccharides, triterpenes, nucleosides, sterols, alkaloids, furans, amino acids, etc., among which the most important medicinal ingredients are Ganoderma triterpenoids, which have anti-inflammatory properties. Tumor, liver protection, immune regulation, blood sugar lowering and other effects. Increasing the content of triterpenoids in Ganoderma lucidum plays an important role in the wide application of Ganoderma lucidum. [0003] Current studies ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/645
CPCC12P33/00
Inventor 胡学博胡延如
Owner HUAZHONG AGRI UNIV
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