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Glossy ganoderma fermentation filtrate by using panax ginseng as culture mediums and application of glossy ganoderma fermentation filtrate to cosmetics

A technology of Ganoderma lucidum fermentation and culture medium, applied in cosmetics, cosmetic preparations, cosmetic preparations, etc., can solve problems such as severe hydrolysis conditions and environmental hazards, achieve mild temperature and pH, ensure safety, and maintain natural activity. Effect

Inactive Publication Date: 2019-05-07
柏玮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, it was mainly obtained by hydrolyzing saponin glycosidic bonds by physical and chemical methods, but the hydrolysis conditions were severe, and pollutants such as organic solvents, acids and alkalis caused great harm to the environment

Method used

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  • Glossy ganoderma fermentation filtrate by using panax ginseng as culture mediums and application of glossy ganoderma fermentation filtrate to cosmetics
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  • Glossy ganoderma fermentation filtrate by using panax ginseng as culture mediums and application of glossy ganoderma fermentation filtrate to cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation and detection of ganoderma lucidum fermentation filtrate with ginseng as medium

[0034] Inoculate 50mL of the amplified fermented Ganoderma lucidum liquid into 500mL medium containing ginseng root powder. The medium components are: 50 mesh ginseng root powder 2%, glucose 6%, corn flour 3%, peanut meal 0.25%, yeast powder 0.1%, peptone 1%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.45%. After culturing in a shaker at 30° C. and 200 rpm for 96 hours, the fermented broth was sterilized by electric shock, and the sterilized fermented broth was centrifuged at 12,000 rpm for 20 min. The collected supernatant is the Ganoderma lucidum fermentation filtrate with ginseng as the medium provided by the present invention.

[0035] The Ganoderma lucidum fermentation filtrate prepared in this example with ginseng as a medium has a viscous liquid appearance and a light yellow, golden yellow to brownish yellow color. pH5.0-6.8, viscosity 100-800cp...

Embodiment 2

[0039] Example 2. Anti-free radical efficacy analysis of ganoderma lucidum fermentation filtrate using ginseng as a medium

[0040] DPPH is an early synthesized organic free radical, which is often used to evaluate the hydrogen-donating ability of antioxidants. It is very stable in organic solvents, has a purple color, and has a characteristic absorption peak at , when it encounters a free radical scavenger , the lone pair of electrons of DPPH is paired to make it fade, that is, the light absorption value at the maximum absorption wavelength becomes smaller. Therefore, the scavenging effect of the sample on DPPH free radicals can be evaluated by measuring the change of the absorbance value.

[0041] The specific experimental steps of the DPPH free radical scavenging experiment are as follows:

[0042] (1) Take an equal volume (usually 3mL) of the test solution and mix it with 2×10-4mol / L DPPH solution (A1 tube);

[0043] (2) Mix an equal volume of absolute ethanol (solvent o...

Embodiment 3

[0048] Example 3, anti-aging test of ganoderma lucidum fermentation filtrate with ginseng as medium

[0049] Subject population: 70 adult female volunteers, aged between 45-55;

[0050] Test method: use the Ganoderma lucidum fermented filtrate with ginseng as the medium prepared in Example 1 in the eye area (comprising the dark circle area of ​​the lower eyelid and the crow's feet area); after 15 minutes and continuous use for 4 weeks, use the FOITS method (skin rapid Optical imaging method) to measure changes in skin texture to evaluate the degree of reduction in depth and length of wrinkles and fine lines in the crow's feet area at the corners of the eyes. The test results are shown in Table 1 below. It can be seen that the fermentation filtrate of Ganoderma lucidum with ginseng as the medium has certain anti-aging effects.

[0051] Table 1 Trial effect test of Ganoderma lucidum fermentation filtrate using ginseng as medium

[0052]

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Abstract

The invention discloses glossy ganoderma fermentation filtrate by using panax ginseng as culture mediums and an application of the glossy ganoderma fermentation filtrate to cosmetics. The fermentationfiltrate is supernatant which is obtained through the steps of mixing effective components of panax ginseng, water, glossy ganoderma mycelia and other nutrient components to obtain an initial system,performing fermentation to obtain fermentation liquid, performing sterilization and performing centrifugation. According to the glossy ganoderma fermentation filtrate disclosed by the invention, thepanax ginseng is subjected to whole plant fermentation through the glossy ganoderma mycelia, so that all the efficacy components and the activity thereof of plants are reserved, and running off of active components, caused by an extraction method, is avoided; the active components in the panax ginseng, such as ginsenoside, are subjected to biotransformation through enzymes in the glossy ganodermamycelia, so that the skin care efficacy of the glossy ganoderma fermentation filtrate is improved. The conditions of fermentation temperature, pH and the like are mild, essence is not added, and the safety of products is guaranteed. Besides, fermentation products have natural fresh medicine fragrance. The product namely the glossy ganoderma fermentation filtrate is rich in active components of theginsenoside, ganoderan, ganoderma triterpenes and the like, can adjust the immunity of skin, and has the skin care functions of preserving moisture and resisting ageing.

Description

technical field [0001] The invention belongs to the field of cosmetics, and relates to a ganoderma lucidum fermentation filtrate using ginseng as a culture medium and its application in cosmetics. Background technique [0002] Ginseng (Panax ginseng C.A. Meyer) is a precious Chinese medicinal material of Araliaceae, known as the king of medicines, and is widely used. Modern pharmacology proves that ginseng has the functions of anti-oxidation, promoting blood circulation, lowering blood sugar, analgesia, anti-cancer and improving immunity. The main functional components in ginseng include: ginsenosides, ginseng polysaccharides, etc. Ginsenosides belong to triterpenoids, which are glycoside compounds formed by linking aglycone and sugar. So far, there are more than 60 kinds of ginsenosides that have been isolated and their structures determined. Most saponin monomers have significant pharmacological effects, and the prospect of new drug development is good, such as Rg1 and ...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/9728A61Q19/00A61Q19/02A61Q19/08
Inventor 不公告发明人
Owner 柏玮
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