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Method for preparing high-purity lucid ganoderma hyphostroma powder through liquid submerged fermentation

A technology of liquid submerged fermentation and mycelium powder, which is applied in the fields of botanical equipment and methods, food preparation, horticulture, etc., can solve the problems of unstable final product quality, low content of active ingredients, long fermentation period, etc., and achieves easy large-scale production. The effect of production, good solubility and fluidity, and uniform mycelial fluid

Inactive Publication Date: 2014-10-08
乳山市华隆生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the existing problems such as long solid fermentation period of ganoderma mycelia, easy bacterial contamination, unstable final product quality, and low content of active ingredients, the present invention adopts a liquid submerged culture method to obtain a mixture of ganoderma mycelia and ganoderma polysaccharides. Liquid, and after concentrated, homogenized, spray-dried, form a kind of Ganoderma lucidum mycelia instant brewing powder which is convenient to eat and comprehensive in nutrition. The method comprises the following steps:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Prepare culture medium

[0034] A: Preparation of slant medium

[0035] According to the mass percentage of glucose 6%, soybean flour 15%, corn flour 10%, wheat flour 10%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.08%, agar 3%, peptone 3%, and the rest is sweet potato extract. Adjust the pH to 7.0, pack into test tubes, mix well, and then sterilize at high temperature. Sterilization conditions: 121~123°C, 30~40min; take out the test tube after 10~15min after sterilization, place it on a slope, and transfer the culture medium on the slope to Cultivate at a constant temperature in an incubator at 28-36°C for 18-20 hours, and take it out for use if no colonies grow out;

[0036] B: Preparation of Shake Flask Medium

[0037] According to the mass percentage of glucose 6%, soybean flour 15%, corn flour 10%, wheat flour 10%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.08%, peptone 3%, and the rest is sweet potato extract, the pH is a...

Embodiment 2

[0058] 1. Prepare culture medium

[0059] A: Preparation of slant medium

[0060] According to the mass percentage of glucose 7%, soybean flour 16%, corn flour 11%, wheat flour 12%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, agar 4%, peptone 5%, and the rest is sweet potato extract. Adjust the pH to 7.0, pack into test tubes, mix well, and then sterilize at high temperature. Sterilization conditions: 121~123°C, 30~40min; take out the test tube after 10~15min after sterilization, place it on a slope, and transfer the culture medium on the slope to Cultivate at a constant temperature in an incubator at 28-36°C for 18-20 hours, and take it out for use if no colonies grow out;

[0061] B: Preparation of Shake Flask Medium

[0062] According to the mass percentage of glucose 7%, soybean flour 16%, corn flour 11%, wheat flour 12%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, peptone 5%, and the rest is sweet potato extract, the pH is adj...

Embodiment 3

[0083] 1. Prepare culture medium

[0084] A: Preparation of slant medium

[0085] According to the mass percentage of glucose 8%, soybean flour 17%, corn flour 12%, wheat flour 13%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.12%, agar 5%, peptone 7%, and the rest is sweet potato extract. Adjust the pH to 7.0, pack into test tubes, mix well, and then sterilize at high temperature. Sterilization conditions: 121~123°C, 30~40min; take out the test tube after 10~15min after sterilization, place it on a slope, and transfer the culture medium on the slope to Cultivate at a constant temperature in an incubator at 28-36°C for 18-20 hours, and take it out for use if no colonies grow out;

[0086] B: Preparation of Shake Flask Medium

[0087] According to the mass percentage of glucose 8%, soybean flour 17%, corn flour 12%, wheat flour 13%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.12%, peptone 7%, and the rest is sweet potato extract, the pH is ...

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Abstract

The invention provides a method for preparing high-purity lucid ganoderma hyphostroma powder through liquid submerged fermentation. The method solves the problems that the existing solid fermentation period of lucid ganoderma hyphostroma is long, the lucid ganoderma hyphostroma is prone to being contaminated by bacteria, the quality of the final product is unstable, and the content of active ingredients is low. The method includes the steps of culture medium preparation, strain activation, inoculation and culture, fermentation expansion, concentration, homogenization, spray drying and the like. The culture medium preparation step includes the procedures of A, agar slant culture medium preparation, B, shake flask culture medium preparation, C, seeding tank culture medium preparation and D, fermentation tank culture medium preparation. The method is widely used for processing lucid ganoderma hyphostroma powder in the food industry.

Description

technical field [0001] The invention relates to a preparation method of health food, in particular to a preparation method of high-purity ganoderma lucidum mycelia powder by submerged liquid fermentation. Background technique [0002] Ganoderma lucidum has good medicinal and health value. In China, its medicinal history has been nearly 2000 years. Modern research has even shown that Ganoderma lucidum mycelia contains polysaccharides, various amino acids, active peptides, triterpenes, stearic acid, various trace elements and alkaloids and other biologically active substances, which have anti-tumor, anti-aging, Various health care values ​​such as radiation protection and immunity improvement; and the mycelium has an enrichment effect, which can convert the inorganic forms of rare elements in the environment such as selenium, germanium, and iodine into organic forms, which is conducive to human body absorption, which further enhances the Efficacy and value of Ganoderma lucidu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A23L1/28A23L1/29A23L33/00
Inventor 张文镨王溢刘天伦
Owner 乳山市华隆生物科技股份有限公司
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