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Ganoderma mycelium fermentation liquor with high ganoderma polysaccharide content and method for preparing ganoderma mycelium fermentation liquor

A technology of Ganoderma lucidum polysaccharide and Ganoderma lucidum mycelium, which is applied in fermentation, fungi and other directions, can solve the problems of long production cycle, high cost, differences in active ingredient composition and content, etc., and achieves the effect of less possibility of pollution and short fermentation time.

Inactive Publication Date: 2018-04-17
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method of ganoderma lucidum mycelia fermentation liquid with high content of ganoderma lucidum polysaccharides, to solve the problem that the ganoderma lucidum cultivation method in the prior art has a long production cycle, needs foster fruiting bodies, high cost, and insufficient yield of ganoderma lucidum polysaccharides High, large-scale production difficulties, and large differences in the composition and content of active ingredients between different batches, etc.

Method used

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  • Ganoderma mycelium fermentation liquor with high ganoderma polysaccharide content and method for preparing ganoderma mycelium fermentation liquor

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preparation example Construction

[0018] The invention relates to a preparation method of a ganoderma lucidum mycelia fermentation liquid with high ganoderma polysaccharide content, comprising:

[0019] The ganoderma lucidum strain is cultured in inclined test tubes to obtain mycelium blocks, and the mycelium blocks are inoculated on plate medium for mycelial culture, and then transferred to malt medium for liquid fermentation culture at 25-29°C for 3-7 days.

[0020] Preferably, when the Ganoderma lucidum strain is Ganoderma lucidum, the culture time is 3 days; when the Ganoderma lucidum strain is Ganoderma lucidum, the culture time is 4 days; days; when the ganoderma lucidum strain is Heizhi, the culture time is 4 days.

[0021] The method adopts a mycelium liquid fermentation method, the ganoderma lucidum polysaccharide content is high, the fermentation method is more environmentally friendly, the cost is lower, the required time is shorter, the fermentation method is simple, easy to operate, suitable for l...

Embodiment

[0043] 1. Preparation of medium and solution

[0044] Plate medium: MEA (Malt Extract Ager): malt extract 30g, soybean peptone 3g, agar malt extract 3w / v%, soybean peptone 0.3w / v%, agar 1.5w / v%, h 2 O 1L to constant volume, pH 5.6, sterilized at 121°C for 15 minutes.

[0045] Malt medium: malt extract powder 2w / v%, glucose 2w / v%, peptone 0.1w / v%, yeast extract 0.1w / v%, H 2 O 1L to constant volume, sterilized at 115°C for 20min.

[0046] CYM medium: glucose 20g, peptone 2g, yeast powder 2g, MgSO 4 ﹒ 7H 2 O0.5g, K 2 HPO 4 1g, KH 2 PO 4 0.46g, H 2 O 1L.

[0047] 2. Liquid fermentation culture method of Ganoderma lucidum strain

[0048] After each strain was cultured in a slant test tube, take a piece of 1cm 2 The mycelium block was inoculated into the plate medium, and cultured at 30°C for 7 days. Then take 1.5cm from the plate medium 2 Inoculate into malt culture medium (300ml Erlenmeyer flask, liquid volume 100ml) and CYM medium respectively, cultivate at 27°C f...

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Abstract

The invention relates to the field of fungus fermentation, in particular to ganoderma mycelium fermentation liquor with a high ganoderma polysaccharide content and a method for preparing the ganodermamycelium fermentation liquor. The method includes carrying out slant tube culture on ganoderma strains to obtain mycelium blocks; inoculating the mycelium blocks to plate culture media, carrying outmycelium culture, then switching the mycelium blocks to malt culture media; carrying out liquid fermentation culture at the temperature of 25-29 DEG C for 3-7 days. The ganoderma mycelium fermentationliquor and the method have the advantages that mycelium liquid fermentation processes are adopted, accordingly, the ganoderma mycelium fermentation liquor is high in ganoderma polysaccharide content,fermentation processes are environmentally friendly and simple and are low in cost, short in required time and convenient to implement, and the ganoderma mycelium fermentation liquor and the method are suitable for large-scale industrial production and convenient to popularize.

Description

technical field [0001] The invention relates to the field of fungal fermentation, in particular to a ganoderma lucidum mycelia fermentation liquid with high ganoderma polysaccharide content and a preparation method thereof. Background technique [0002] Ganoderma lucidum [(Leyss.ex.Fr.) Karst] belongs to Basidiomycetes, Polyporaceae, Ganoderma higher fungi. Ganoderma lucidum has a history of thousands of years in our country, as early as in the "Shen Nong's Materia Medica", it is recorded that Ganoderma lucidum strengthens the foundation and nourishes the vitality, long-term food will not grow old, light body and prolong life. "Compendium of Materia Medica" listed it as a top-grade medicine, pointing out that Chizhi "bitter, flat, non-toxic, mainly treats knots in the chest, nourishes the heart, nourishes the middle, increases wisdom, and does not forget", and Zizhi "non-toxic, good color, cures fatigue, cure hemorrhoids". [0003] The active substances contained in Ganode...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04
CPCC12N1/14C12P19/04
Inventor 冯湘沅彭源德谢纯良龚文兵朱作华段盛文成莉凤刘志远杨琦郑科
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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