Method for detecting glossy ganoderma spore oil content in glossy ganoderma oil preparation

A technology of ganoderma spore oil and detection method, which is applied in the detection field of ganoderma spore oil content in ganoderma spore oil preparations, can solve the problems of unfavorable control and monitoring of drug quality, poor reproducibility of measurement results, unstable measurement results, etc. No interference, perfect quality standard, high recovery effect

Active Publication Date: 2007-06-27
江苏海王健康生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this method adopts ursolic acid as a reference substance in the assay process of total triterpenoids, but in the research results of the triterpenoid components of Ganoderma lucidum, Ganoderma lucidum spore powder, and Ganoderma spore oil, no ursolic acid is found to exist, In the determination process, it is more appropriate to use the representative components of the triterpenes as reference substances; Determination, in the determination process, along with the time of color development, the temperature of color development, the time of determination etc., the difference of the measurement results is relatively large, the reproducibility of the measurement results is poor, and the measurement results are unstable; During the determination of triterpenoids, the total triterpenes were not separated, and Ganoderma lucidum spore oil was directly used to oxidize color with perchloric acid, and a large number of long-chain aliphatic hydrocarbons in Ganoderma lucidum spore oil could also be detected by high chloride Acid oxidizes and develops color, thus causing false positive results in the determination. Using this method to determine other edible vegetable oils can also detect higher levels, which cannot form a one-to-one correspondence with the content of triterpenoids in Ganoderma lucidum spore oil in drugs. Ganoderma lucidum spore oil is directly oxidized with perchloric acid to detect the content of triterpenoids in the ganoderma spore oil preparation. It is easily interfered by other components of the ganoderma spore oil, which is not conducive to the control and monitoring of drug quality in production.

Method used

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  • Method for detecting glossy ganoderma spore oil content in glossy ganoderma oil preparation
  • Method for detecting glossy ganoderma spore oil content in glossy ganoderma oil preparation
  • Method for detecting glossy ganoderma spore oil content in glossy ganoderma oil preparation

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Embodiment 1

[0030] Example 1 Determination of Ganoderma acid E content in Ganoderma lucidum spore oil

[0031] (1) Instruments and reagents

[0032] High-performance liquid chromatography, including Waters1525 pump, 2487 dual-channel ultraviolet detector, Waters column thermostat, Breeze liquid chromatography workstation, Brasson ultrasonic cleaner. Ganoderma acid E reference substance (extracted and isolated from Ganoderma lucidum spores, tested by mp, IR, UV, MS, 1 H-NMR etc. are identified as ganoderma acid E, HPLC normalization method measures and records the purity and is 99.6%), acetonitrile is U.S. Tedia chromatographically pure, water is ultrapure water, and other reagents are analytically pure. The drug samples to be tested were Ganoderma lucidum spore oil soft capsules (batch numbers: 051114, 051217, 060123).

[0033] (2) Chromatographic conditions

[0034] Chromatographic column: Kromasil C18, 4.6 mm×250 mm, 5 μm; column temperature: 30° C.; mobile phase: acetonitrile-0.1% f...

Embodiment 2

[0074] Determination of total ganoderma acid content in embodiment 2 ganoderma lucidum spore oil

[0075] (1) Instruments and reagents

[0076] UV Agilent8453 ultraviolet-visible spectrophotometer (Agilent Technologies, Inc., USA). The drug samples to be tested were Ganoderma lucidum spore oil soft capsules (batch numbers: 051114, 051217, 060123), and Ganoderma acid E reference substance (self-made, with a purity of 99.6%); all reagents used were of analytical grade.

[0077] (2) Preparation of reference substance solution

[0078] Accurately weigh 5.36mg of Ganoderma acid E reference substance, put it in a 25ml measuring bottle, add saturated NaHCO 3 The solution was dissolved and diluted to the mark as the reference solution.

[0079] (3) preparation of test solution

[0080] Take 3g of the content of the test product, weigh it accurately, add 50ml of petroleum ether to dissolve, put it on the treated silica gel column (8g, 18mm×400mm), add 150ml of ethyl acetate: petrol...

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Abstract

The measuring method measures contents of general ganoderma lucidum acid, ganoderma lucidum acid E in spore oil preparation of ganoderma lucidum. The testing standard is as following: in each 1.0g of spore oil preparation of ganoderma lucidum (equivalent to 5g of crude drugs of ganoderma lucidum spore), general ganoderma lucidum acid contained is not allowed less than 1.0mg counted by ganoderma lucidum acid E (C30H54O7); content of ganoderma lucidum acid E is between 0.001mg-0.050mg, i.e. content of ganoderma lucidum acid E is between 0.010%-0.50%. The invention makes stable testing and controlling quality of spore oil preparation of ganoderma lucidum.

Description

technical field [0001] The invention relates to the field of drug detection methods, in particular to a method for detecting the content of ganoderma lucidum spore oil in ganoderma spore oil preparations. Background technique [0002] Ganoderma lucidium spores are extremely small spores ejected from the cap during the mature growth stage of Ganoderma lucidum. They are the reproductive cells of Ganoderma lucidum and have all the genetically active substances of Ganoderma lucidum. The pharmacological effects of Ganoderma lucidum spores mainly include anti-tumor, immune regulation, regulation of blood lipids, and regulation and improvement of the nervous, cardiovascular and respiratory systems. Ganoderma lucidum spore oil is an oily extract obtained by breaking the wall of Ganoderma lucidum spores by supercritical extraction or organic solvent extraction. The chemical composition of Ganoderma lucidum spore oil is complex, mainly containing oils, fatty acids, sterols and triter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N1/28G01N30/86
Inventor 吴敏李颖黄光华
Owner 江苏海王健康生物科技有限公司
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