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Quality evaluation method for ganoderma lucidum medicinal material

A quality evaluation and technology of red ganoderma lucidum, applied in the field of quality control of red ganoderma lucidum medicinal materials, can solve the problems of rare and expensive reference substances, unable to represent the content of ganoderma lucidum acid, difficult production and circulation, etc., to facilitate identification, ensure consistency, and guarantee The effect of accuracy

Active Publication Date: 2017-01-18
GUANGZHOU SHENNONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the reference substance is too rare and expensive, and the content of a single type of ganoderma acid cannot represent the content of all ganoderma acids in ganoderma
Therefore, it is difficult to apply to the field of production and circulation

Method used

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  • Quality evaluation method for ganoderma lucidum medicinal material
  • Quality evaluation method for ganoderma lucidum medicinal material
  • Quality evaluation method for ganoderma lucidum medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of Ganoderma lucidum extract reference substance

[0047] 1.1. Experimental overview

[0048] The solvent used in the preparation of the Ganoderma lucidum extract is methanol, and the extraction method is heating and refluxing.

[0049] 1.2. Experimental method

[0050] Two kinds of medicinal materials that were identified as red Ganoderma lucidum were separately prepared into Ganoderma lucidum extract. The preparation method is as follows: weigh 2kg of red Ganoderma lucidum medicinal material and crush, add 8 times the amount of methanol 16L, heat reflux and extract twice, each time 1 Hours (liquid boiling starts timing), filter, collect the filtrate, concentrate to 5L at 65°C under reduced pressure, add 120g of silica gel as auxiliary material, and continue to concentrate to dryness to obtain 200g of Ganoderma lucidum extract, respectively numbered No. 1 Ganoderma lucidum extract, No. 2 red Ganoderma lucidum extract.

[0051] Weigh 200 g of No. 1 Ganoder...

Embodiment 2

[0052] Example 2 Calibration of Ganoderma lucidum extract reference substance

[0053] 2.1 Experimental overview

[0054] Using ganoderic acid A, ganoderic acid B, ganoderic acid C2 and ganoderic acid G as reference materials, the content of ganoderic acid A, ganoderic acid B, ganoderic acid C2 and ganoderic acid G in the reference substance of Ganoderma lucidum extract was calibrated by liquid chromatography . The calibration method is as follows:

[0055] 2.2 Reagents

[0056] Ganoderma acid A, Ganoderma acid B, Ganoderma acid C2, Ganoderma acid G were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0057] 2.3 Sample preparation of Ganoderma lucidum extract reference substance and ganoderic acid reference substance

[0058] Weigh 0.2g of Ganoderma lucidum extract reference substance, dissolve it with methanol and dilute to a 2ml volumetric flask. The sample is obtained by passing the 0.22um microporous membrane.

[0059] Weigh 5 mg of ganoderic acid A, ganoderic acid B, gano...

Embodiment 3

[0069] Example 3 The ultraviolet absorption analysis of the main components of the reference substance of the Ganoderma lucidum extract (retention time 15-65min)

[0070] 3.1 Experimental overview

[0071] The reference substance of Ganoderma lucidum extract was determined by liquid chromatography, and the ultraviolet absorption of the peak with the retention time of 15-65min in the liquid chromatography in the spectrum was analyzed.

[0072] 3.2 Sample preparation

[0073] Weigh 0.2g of Ganoderma lucidum extract reference substance, dissolve it with methanol and dilute to a 2ml volumetric flask. The sample is obtained by passing the 0.22um microporous membrane.

[0074] 3.3 Liquid chromatography conditions

[0075] Column: TSK gel ODS-80Tm (5μm 150*4.6mm)

[0076] Mobile phase: the following ratio, gradient elution

[0077]

[0078]

[0079] Flow rate: 1.2mL / min; Column temperature: 25℃; Detection wavelength: 254nm Injection volume: 10μl

[0080] 3.4 Experimental results:

[0081] Analyze ...

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Abstract

The invention provides a quality evaluation method for a ganoderma lucidum medicinal material. The quality evaluation method comprises the following steps: taking a ganoderma lucidum extract comparison product as comparison, and measuring a ganoderma lucidum medicinal material sample to be measured by liquid chromatography at any wavelength in a detecting wavelength range of 210 to 280 nm; summing all ultraviolet absorption peak areas of which the retention time is in the interval of 15 to 65 minutes in an obtained liquid chromatogram, and comparing the sum with an ultraviolet absorption peak area corresponding to ganoderic acid A in the comparison product; calculating the content of total ganoderma triterpenes in the ganoderma lucidum medicinal material sample to be measured; taking the content of the total ganoderma triterpenes as a quality index for evaluating the quality of the ganoderma lucidum medicinal material. According to the quality evaluation method for the ganoderma lucidum medicinal material provided by the invention, the quality of the ganoderma lucidum can be evaluated more accurately, comprehensively and conveniently, and the detection cost can also be reduced.

Description

Technical field [0001] The invention belongs to the field of quality control of medicinal materials, and particularly relates to a quality control method of red ganoderma lucidum medicinal materials. Background technique [0002] Ganoderma lucidum has a history of thousands of years in our country. It is recorded in the "Shen Nong's Materia Medica" as early as that Ganoderma lucidum solidifies the root and cultivates its vitality. [0003] However, as a kind of fungal medicinal material, Ganoderma lucidum has many varieties. The chemical composition of different species of Ganoderma lucidum is very different. Even if the same is red Ganoderma lucidum, it is divided into many subspecies. The fruit body and spore powder composition of different parts of Ganoderma lucidum are completely different. The silk body is also very different from the fruit body in composition. The efficacy of Chinese medicine is based on the stability of the composition and content of the chemical substances...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/90
Inventor 李莹雪李乾坤王锐银黄静贤李紫薇
Owner GUANGZHOU SHENNONG BIOLOGICAL TECH
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