4 results about "Dammarenediol" patented technology

The application belongs to the technical field of genetic engineering, and discloses a kind of saccharomyces cerevisiae for producing rare ginsenoside by seaweed biomass and a construction method and application thereof.The saccharomyces cerevisiae has the following characteristics: overexpression of agarase, neojuncanohydrolase, hydroxymethylglutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammaradienol synthase, protopanaxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyltransferase.The application combines enzymolysis of seaweed biomass with fermentation of rare ginsenoside, which not only endows wild-type yeast with the ability to degrade seaweed biomass that it originally does not have, but also effectively improves the yield of squalene and downstream terpenes (rare ginsenoside Rh1) by overexpression of tHMG1 and IDI1.The saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, and has the characteristics of simplicity, economy and ecological friendliness, can convert cheap biomass into high-value products, and provides a way for the development of marine bioeconomy.

The application discloses a method for producing dammaradien-diol-II glycoside 3beta-O-Glc-DM and a construction method of a recombinant bacterium, the recombinant bacterium obtained by the method, and application of the recombinant bacterium in preparation of 3beta-O-Glc-DM.

The invention relates to a method for preparing dammarendiol in nicotiana benthamiana and teasel, and belongs to the technical field of biology. The method comprises the following steps: inserting a target gene into a transient expression vector to obtain a recombinant vector; the target gene is as shown in SEQ ID NO. 1; the method comprises the following steps: by taking leaves of seedlings of nicotiana benthamiana and teasel as materials, infecting GV3101 / pEAQ-HT-DDS agrobacterium liquid, culturing, and extracting the cultured plant leaves, thereby obtaining dammarendiol. According to the method, tobacco and teasel leaves are infected by using an agrobacterium tumefaciens vacuum infiltration method, and a safe and efficient transient expression system which is easy for large-scale production of dammarendiol is established in the tobacco and teasel leaves.

The application discloses a dammaradiene synthase and application thereof, and belongs to the technical field of bioengineering.The application aims to provide a protein with dammaradiene synthase and a key amino acid site capable of transforming a triterpene cyclase OSC protein into a catalytic active site for synthesizing dammaradiene.The application provides a dammaradiene synthase, and the amino acid sequence of the dammaradiene synthase is shown as SEQ ID NO.3.It is proved by a site-directed mutation experiment that the mutation of Y260N of the homologous gene of the Panax notoginseng dammaradiene synthase OeOSC14 can make the OSC catalyze the efficient synthesis of dammaradiene from squalene.