Cultivation method of novel dammarane-type ginsenoside-containing paddy rice variety

Abstract

The invention discloses a cultivation method of a novel dammarane-type ginsenoside-containing paddy rice variety and belongs to the field of genetic engineering. The breeding method is characterized in that a full-length cDNA of a gene of a dammarenediol synthetase as a key enzyme of dammarane-type ginsenoside biosynthesis is constructed to double-T-DNA plant expression vectors; the constructed product is subjected to genetic transformation in paddy rice; and through screening cultivation, the novel dammarane-type ginsenoside-containing paddy rice variety is obtained. A test proves that the novel dammarane-type ginsenoside-containing paddy rice variety comprises 0.020% of 20(S)-protopanoxadiol (PPD) and 0.173% of 20(S)-protopanaxatriol (PPT). The cultivation method can be used for cultivation of high-quality ginseng rice varieties containing dammarane-type ginsenoside, can improve a health care value of rice as a main food of a human body, can widen a ginsenoside source, can alleviate the scarcity of a ginsenoside drug source, and has a good application prospect.

Description

Technical field [0001] The present invention belongs to the field of crop gene engineering. Specifically, it is a creation method that involves rice containing tagane -type ginseng saponin. Background technique [0002] Rice is one of the main food crops in the world. Nearly half of the world has rice as a staple food.Traditional breeding technology has made great contributions to the improvement of rice quality, but because it is difficult to overcome the obstacles of reproductive isolation between species, the health quality of rice quality, especially rice, is difficult to improve.The rapid development of modern genetic engineering technology provides an effective means for the genetic improvement of rice quality, that is, through genetic engineering methods, new metabolites can be synthesized into rice, making rice synthesize new functional ingredients, thereby improving riceNutritional health value. [0003] Ginseng ( Panax ginseng C.A.Mey.) PANAX ) Plants are one of the tra...

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Problems solved by technology

But so far there is no literature report on the transformation of Panax dammarenediol synthase gene into rice and the creation of new rice germplasm containing dammarane-type ginsenosides

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