4 results about "Hydroxy methyl glutaryl" patented technology

The application belongs to the technical field of genetic engineering, and discloses a kind of saccharomyces cerevisiae for producing rare ginsenoside by seaweed biomass and a construction method and application thereof.The saccharomyces cerevisiae has the following characteristics: overexpression of agarase, neojuncanohydrolase, hydroxymethylglutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammaradienol synthase, protopanaxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyltransferase.The application combines enzymolysis of seaweed biomass with fermentation of rare ginsenoside, which not only endows wild-type yeast with the ability to degrade seaweed biomass that it originally does not have, but also effectively improves the yield of squalene and downstream terpenes (rare ginsenoside Rh1) by overexpression of tHMG1 and IDI1.The saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, and has the characteristics of simplicity, economy and ecological friendliness, can convert cheap biomass into high-value products, and provides a way for the development of marine bioeconomy.

The invention belongs to the technical field of genetic engineering, and discloses saccharomyces cerevisiae for producing rare ginsenoside by utilizing seaweed biomass as well as a construction method and application of the saccharomyces cerevisiae. The saccharomyces cerevisiae has the following characteristics: overexpression agarase, neoagarobiose hydrolase, hydroxymethyl glutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammarendiol synthase, protopanoxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyl transferase. According to the invention, seaweed biomass enzymolysis and rare ginsenoside fermentation are combined, so that wild yeast is endowed with the capability of degrading seaweed biomass, which does not exist originally; the overexpression of tHMG1 and IDI1 effectively increases the yield of squalene and downstream terpenoids (rare ginsenoside Rh1). The Saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, does not have the characteristics of simplicity, economy and eco-friendliness, can convert cheap biomass into products with high added value, and provides a way for developing marine biological economy.

The invention discloses a recombinant saccharomyces cerevisiae engineering bacterium which carries a gene expression cassette containing a hydroxymethyl glutaryl-coenzyme A reductase coding gene and an isoprene synthetase coding gene, and discloses the recombinant saccharomyces cerevisiae engineering bacterium which is constructed by adopting the engineering bacterium and is used for producing dammarendiol. The invention discloses a dammarendiol gene carrier which carries a gene expression cassette containing a dammarendiol synthetase gene, and further discloses a method for producing dammarendiol. According to the saccharomyces cerevisiae engineering strain constructed through synthetic biology and genetic engineering technical means, the dammarendiol synthetase can be efficiently recombined and expressed, then a precursor, namely the dammarendiol, of ginsenoside protopanoxadiol is synthesized, and a foundation is laid for finally achieving ginsenoside biosynthesis and large-scale industrial production.

The application provides a saccharomyces cerevisiae engineering bacterium for producing squalene through endogenous and artificial synthetic pathways and an application thereof, the saccharomyces cerevisiae engineering bacterium takes ZS00 strain as a starting strain, overexpresses acetyl coenzyme A acetyltransferase gene, hydroxymethylglutaryl coenzyme A synthase gene and N-terminal truncated hydroxymethylglutaryl coenzyme A reductase gene, and effectively improves the yield of squalene. Heterologous expression of mevalonate kinase gene and mevalonate pyrophosphate decarboxylase gene, overexpression of the key gene squalene synthase gene for promoting the conversion of precursors into squalene effectively promotes the synthesis of squalene. The introduction of isopentenol utilization pathway genes and their mutants, through the regulation of IU pathway key substrate, overexpression of isopentenyl pyrophosphate isomerase gene and farnesyl pyrophosphate synthase gene, the final strain can effectively accumulate squalene to 687.93 mg / L. The application realizes the breakthrough of squalene yield and efficiency through complementation and synergistic effect.