The invention discloses an antistatic high-cleanliness latex glove and a preparation method thereof. The anti-static waterproof coating is prepared from the following raw materials in parts by weight: 50 to 100 parts of latex, 20 to 40 parts of butyronitrile, 20 to 40 parts of polyvinyl chloride, 10 to 20 parts of sulfur, 10 to 20 parts of a conductive outer layer, 30 to 60 parts of a leather material, 10 to 20 parts of an anti-static PC (Polycarbonate) board, 20 to 40 parts of carbon black, 20 to 40 parts of mica powder, 5 to 10 parts of a de-foaming agent, 10 to 20 parts of wool fat, 10 to 20 parts of an accelerant, 10 to 20 parts of an anti-aging agent and 5 to 10 parts of a thickening agent. The preparation method comprises the following steps: S1, firstly, adding latex, butyronitrile, polyvinyl chloride, sulfur and a leather material into a stirrer, stirring and mixing according to fixed time, obtaining a first mixture after mixing is finished, meanwhile, putting the first mixture into a standing box, standing, and mixing, so as to obtain a second mixture; the antistatic high-cleanliness latex glove is good in antistatic capacity, long in service life, convenient to produce and convenient to popularize and use.