Microencapsulation preparation method of rhodococcus B7740 carotenoids

A carotenoid and Rhodococcus technology, which is applied in the directions of food preparation, food forming, application, etc., can solve the problems of easy oxidative degradation, light irradiation, instability and sensitivity of carotenoids, and achieves avoiding adverse effects, reducing production costs, and achieving high yields. rate effect

Active Publication Date: 2015-04-22
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Microencapsulation technology solves the problems of instability, easy oxidative degradation and sensitivity to light in the commercial production of carotenoids

Method used

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  • Microencapsulation preparation method of rhodococcus B7740 carotenoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for preparing rhodococcus B7740 carotenoid microcapsules, the steps are:

[0037] A. Weigh 0.5g of Rhodococcus B7740 freeze-dried bacterial powder (provided by Zhejiang Wanli University) and mix it with 10mL of 1mg / mL lysozyme (20000U / mg; biochemical reagent Sinopharm Chemical Reagent Co., Ltd.), break the cell wall in a 37°C water bath for 1 hour, and add 10mL Mix with saturated zinc acetate to precipitate protein, centrifuge at 6000r / min for 10min, discard the supernatant, and extract once or twice or three times with methanol / dichloromethane (8:2, v / v) at room temperature (20-25°C). A carotenoid extract is obtained.

[0038] B. Rotary evaporation of the carotenoid extract until viscous, adding 10 mL of soybean oil, and continued rotary evaporation to remove the organic solvent.

[0039]C. Weigh 1.5g chitosan and 3g gum arabic respectively, stir the gum arabic in hot water at 60°C to dissolve, cool to 20°C, adjust the pH to 3.6 with glacial acetic acid, and ...

Embodiment 2

[0046] A method for preparing microcapsules of rhodococcus carotenoid extract, the steps of which are:

[0047] A. Weigh 0.5g of Rhodococcus B7740 freeze-dried bacterial powder (provided by Zhejiang Wanli University) and mix it with 10mL 1mg / mL lysozyme (20000U / mg; Biochemical Reagent Sinopharm Chemical Reagent Co., Ltd.), break the cell wall in 37℃ water bath for 1h, add Mix 10mL saturated zinc acetate to precipitate protein, centrifuge at 6000r / min for 10min, discard the supernatant, and extract once or twice or three times with methanol / dichloromethane (8:2, v / v) at room temperature (20-25°C) , to obtain a carotenoid extract.

[0048] B. Rotary evaporation of the carotenoid extract until viscous, adding 10 mL of soybean oil, and continued rotary evaporation to remove the organic solvent.

[0049] C. Weigh 1g of chitosan and 3g of gum arabic respectively, stir the gum arabic in hot water at 60°C to dissolve it, cool to 20°C, adjust the pH to 3.6 with glacial acetic acid, an...

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Abstract

The invention discloses a microencapsulation preparation method of rhodococcus B7740 carotenoids. The method comprises the steps that (1) the rhodococcus carotenoids are extracted through an enzymic method; (2) rhodococcus B7740 carotenoid vegetable oil is prepared; (3) according to the mass ratio of chitosan to Arabic gum, two types of wall material powder are weighed, stirred in hot water to be dissolved and cooled; (4) according to the ratio of the masses of the wall material powder to the volume of the carotenoid vegetable oil, the carotenoid vegetable oil solution is absorbed and homogenized to obtain an o/w type lactescence; (5) a chitosan solution is added and continues to be stirred; (6) the mixture is cooled, curing agent glutaraldehyde is added, the PH is regulated through a NaOH solution, and the mixture continues to be stirred; (7) standing is carried out under the light shading condition, supernate is removed, sediments are washed, and vacuum freezing drying is carried out. The method is easy and convenient to implement, effectively reduces degradation of the carotenoids in the extraction process, reduces carotenoid degradation impurities in core materials and guarantees the physiological activity of carotenoid microcapsules, and the extraction ratio of the carotenoids reaches above 90%.

Description

technical field [0001] The invention belongs to the technical field of processing functional active ingredients, and more specifically relates to a method for preparing microcapsules of carotenoids derived from polar marine oligotrophic bacteria. Background technique [0002] Carotenoids widely exist in nature and have the functions of anti-oxidation, dyeing, prevention and treatment of VA deficiency, enhancement of immunity, and anti-cancer and anti-cancer functions. Many tissues and organs in the human body have carotenoids, but the human body cannot synthesize them by itself and can only ingest them from the outside world. With the increasing awareness and demand for the importance of carotenoids in modern people, the commercialization of carotenoids has become a reality. [0003] At present, commercially available carotenoid products are mainly derived from plants and chemical synthesis. However, with the increase of demand, the low-cost, high-purity, and safer microbi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23P1/04A23L33/00
Inventor 孙智达汪荣
Owner HUAZHONG AGRI UNIV
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