Method for extracting ascorbic acid from vegetables and fruits

A technology for ascorbic acid, vegetables and fruits, applied in the field of analytical chemistry, can solve the problems of inactivation of ascorbic acid, loss of nutritional value, instability of ascorbic acid, etc., and achieve the effects of improving extraction rate and reducing oxidation

Inactive Publication Date: 2011-04-20
FUDAN UNIV
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Problems solved by technology

[0005] Research also shows that ascorbic acid is unstable, and under the condition of heat, it is easily oxidized by the oxygen in the air, and undergoes a series of complex degradation reactions such as dehydration, decarbonation, ring opening, and ring closing, and loses its nutritional value.
The enediol group in the ascorbic acid m

Method used

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  • Method for extracting ascorbic acid from vegetables and fruits
  • Method for extracting ascorbic acid from vegetables and fruits
  • Method for extracting ascorbic acid from vegetables and fruits

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Embodiment 1

[0028] Embodiment 1: NPMAE-HPLC analyzes the content of ascorbic acid in different fruits and vegetables

[0029] Instrument: Shimadzu CL-10 liquid chromatograph (including SPD-10A ultraviolet detector and CTO-10A column thermostat, 20μL manual injection system, chromatography workstation), LWMC-205 adjustable power microwave chemical reactor, AP-9901S vacuum pump; TGL-16G desktop centrifuge; Millipore Direct-Q pure water generator.

[0030] Materials: All kinds of fresh fruits and vegetables. Metaphosphoric acid (analytical pure, Sinopharm Chemical Reagent Co., Ltd.), hydrochloric acid (analytical pure, Sinopharm Chemical Reagent Co., Ltd.), glacial acetic acid (analytical pure, Sinopharm Chemical Reagent Co., Ltd.), methanol (chromatographic pure, Jiangsu Hanbang Technology Co., Ltd.), ascorbic acid (Sigma, St.Louis, MO, USA), and deionized pure water were produced by a Millipore Direct-Q purified water generator.

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Abstract

The invention belongs to the technical field of analysis chemistry, specifically to a method for extracting ascorbic acid from vegetables and fruits by using a nitrogen protection micro wave-assisted extracting technology. The method comprises the following steps of: taking fresh vegetables and fruits, adding a weak acid solution with certain concentration in 1:6-1:12 g/ml times of volume as an extracting solvent, discharging the oxygen in the system via a nitrogen protecting device, extracting at a micro wave condition with certain power for a certain time, cooling, compensating weight, uniformly mixing, centrifuging, taking out supernatant fluid, diluting, analyzing and detecting the content of the ascorbic acid in the vegetables and fruits via high performance liquid chromatography. According to the method, nitrogen protection is combined with micro wave-assisted extraction to discharge the oxygen in the system, thus the oxidization of the ascorbic acid is reduced and the extracting rate is greatly improved. The method is combined with the high performance liquid chromatography for exactly detecting the content of the ascorbic acid in the vegetables and fruits, and is applicable to extracting and separating other components which are easy to oxidize by heating.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for extracting ascorbic acid from fruits and vegetables, in particular to a method for extracting ascorbic acid from fruits and vegetables by utilizing a nitrogen-protected microwave-assisted extraction technology. Background technique [0002] Analytical chemistry pretreatment technology is always an important part of instrumental analysis experiments. Especially for samples with many and complex components such as food and traditional Chinese medicine, how to extract the desired compounds efficiently and quickly is a key issue. [0003] Microwave-assisted extraction (Microwave-assisted Extraction, MAE) is a series of techniques for extracting soluble materials in plants with microwave energy, which is mainly carried out in organic solvents. Research has shown that microwave has a strong penetrating power, can heat uniformly and rapidly inside and outside the material...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 段更利李嫣郁颖佳陈斌段昊天
Owner FUDAN UNIV
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