The invention discloses a preparation method and an application of an ethanol extract of laminarin. The ethanol extract of laminarin is obtained through the preparation method which comprises the following steps: 1, crushing sea tangle, adding an ethanol solution to the crushed sea tangle, extracting for 2-5h at 50-80DEG C; 2, filtering to remove a residue, and adding active carbon to an extract to decolor and remove a fishy smell; and 3, filtering, and concentrating to obtain the ethanol extract of laminarin. The ethanol extract of laminarin is added to cut tobaccos or filter tips in a sprinkling mode, and the mass of the ethanol extract of laminarin accounts for 0.01-1.0% of the mass of cut tobaccos, so the content of free radicals in a mainstream smoke of cigarettes is effectively reduced, the reduction amplitude of the content of the free radicals is more than 15%, and the smoking quality of the cigarettes is improved, thereby the ethanol extract of laminarin, which has irritationreduction effects on nasal cavities, throats and the like, allows the smoke to be smooth, the taste to be soft, the fragrance to be kept, and no side effects to be possessed. The ethanol extract of laminarin has the advantages of no obviously fishy smell, easy obtaining, and low cost.