Preparation method of artificial fullerene-carried nucleus pulposus
A technology of fullerene and nucleus pulposus, which is applied in the field of preparation of fullerene-loaded artificial nucleus pulposus formula, to prevent gel displacement, reduce free radical content, and slow down intervertebral disc degeneration
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[0034] A kind of preparation method of carrying fullerene artificial nucleus pulposus formula, the preparation method of this carrying fullerene artificial nucleus pulposus formula comprises the following steps:
[0035] S1. Preparation of chitosan / type II collagen / β-sodium glycerophosphate / hydroxyethyl cellulose xerogel:
[0036] 1. Preparation of chitosan solution: 1. Selection of materials: Use a 50mL wide-mouth beaker to measure some dilute acetic acid with a concentration of 0.1M and use a balance to measure a certain amount of chitosan with a deacetylation degree greater than 90%. Weighing of sugar powder; 2. Mixing of materials: Use a magnetic stirrer to stir some dilute acetic acid measured inside the beaker, and add the weighed chitosan powder to the inside of the beaker and mix it with the dilute acetic acid inside until the shell After the concentration of the polysan solution is 10-20wt%, stop the stirring and mixing step; 3. Disinfection of the material: place the...
Embodiment 1
[0053] 1. Material preparation
[0054] The balance takes a certain amount of chitosan powder with a degree of deacetylation greater than 90% for subsequent use, and measures some concentrations of 0.1 M dilute acetic acid in a 50mL wide-mouth beaker for subsequent use, and stirs the weighed chitosan with a magnetic stirrer. Stir in the prepared dilute acetic acid that sugar powder is added step by step for about 3 hours, until the chitosan solution becomes clear and translucent, finally obtain the chitosan solution with a concentration of about 15wt%, place the chitosan solution that is stirred uniformly at 121 ℃ in a high temperature and high pressure sterilizing pot for 20 minutes, and after the temperature of the sterilized chitosan solution dropped to room temperature, it was stored in a 4 ℃ refrigerator for later use.
[0055] In the ultra-clean bench, prepare a certain amount of dilute acetic acid with a concentration of 0.02M, filter and sterilize with a 0.22μm filter ...
Embodiment 2
[0068] The process method of this embodiment is the same as that of Example 1, and the anti-inflammatory ability of the prepared fullerene-loaded artificial nucleus pulposus is characterized. LPS lipopolysaccharide was used to construct the macrophage RAW264.7 inflammatory cell model, and then the artificial nucleus pulposus without fullerene and the artificial nucleus pulposus loaded with fullerene were co-cultured with the same number of inflammatory cells. After culturing for 1, 2 and 3 days, use the ELASA kit to detect the contents of TNF-α and IL-6, and make histograms.
[0069] Anti-inflammatory test results such as image 3 As shown, the contents of TNF-α and IL-6 in the fullerene-loaded artificial nucleus pulposus were significantly lower than those in the non-fullerene-added group, which indicated that the fullerene-loaded artificial nucleus pulposus exerted anti-inflammatory effects and reduced the RAW264.7 Inflammatory levels of macrophages.
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