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Preparation method of artificial fullerene-carried nucleus pulposus

A technology of fullerene and nucleus pulposus, which is applied in the field of preparation of fullerene-loaded artificial nucleus pulposus formula, to prevent gel displacement, reduce free radical content, and slow down intervertebral disc degeneration

Active Publication Date: 2018-09-07
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the scavenging of oxygen free radicals in the nucleus pulposus, and there are no reports or applications of artificial nucleus pulposus that can have anti-inflammatory effects.

Method used

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  • Preparation method of artificial fullerene-carried nucleus pulposus
  • Preparation method of artificial fullerene-carried nucleus pulposus
  • Preparation method of artificial fullerene-carried nucleus pulposus

Examples

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preparation example Construction

[0034] A kind of preparation method of carrying fullerene artificial nucleus pulposus formula, the preparation method of this carrying fullerene artificial nucleus pulposus formula comprises the following steps:

[0035] S1. Preparation of chitosan / type II collagen / β-sodium glycerophosphate / hydroxyethyl cellulose xerogel:

[0036] 1. Preparation of chitosan solution: 1. Selection of materials: Use a 50mL wide-mouth beaker to measure some dilute acetic acid with a concentration of 0.1M and use a balance to measure a certain amount of chitosan with a deacetylation degree greater than 90%. Weighing of sugar powder; 2. Mixing of materials: Use a magnetic stirrer to stir some dilute acetic acid measured inside the beaker, and add the weighed chitosan powder to the inside of the beaker and mix it with the dilute acetic acid inside until the shell After the concentration of the polysan solution is 10-20wt%, stop the stirring and mixing step; 3. Disinfection of the material: place the...

Embodiment 1

[0053] 1. Material preparation

[0054] The balance takes a certain amount of chitosan powder with a degree of deacetylation greater than 90% for subsequent use, and measures some concentrations of 0.1 M dilute acetic acid in a 50mL wide-mouth beaker for subsequent use, and stirs the weighed chitosan with a magnetic stirrer. Stir in the prepared dilute acetic acid that sugar powder is added step by step for about 3 hours, until the chitosan solution becomes clear and translucent, finally obtain the chitosan solution with a concentration of about 15wt%, place the chitosan solution that is stirred uniformly at 121 ℃ in a high temperature and high pressure sterilizing pot for 20 minutes, and after the temperature of the sterilized chitosan solution dropped to room temperature, it was stored in a 4 ℃ refrigerator for later use.

[0055] In the ultra-clean bench, prepare a certain amount of dilute acetic acid with a concentration of 0.02M, filter and sterilize with a 0.22μm filter ...

Embodiment 2

[0068] The process method of this embodiment is the same as that of Example 1, and the anti-inflammatory ability of the prepared fullerene-loaded artificial nucleus pulposus is characterized. LPS lipopolysaccharide was used to construct the macrophage RAW264.7 inflammatory cell model, and then the artificial nucleus pulposus without fullerene and the artificial nucleus pulposus loaded with fullerene were co-cultured with the same number of inflammatory cells. After culturing for 1, 2 and 3 days, use the ELASA kit to detect the contents of TNF-α and IL-6, and make histograms.

[0069] Anti-inflammatory test results such as image 3 As shown, the contents of TNF-α and IL-6 in the fullerene-loaded artificial nucleus pulposus were significantly lower than those in the non-fullerene-added group, which indicated that the fullerene-loaded artificial nucleus pulposus exerted anti-inflammatory effects and reduced the RAW264.7 Inflammatory levels of macrophages.

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Abstract

The invention relates to the preparation field of artificial nucleus pulposus, in particular to a preparation method of artificial fullerene-carried nucleus pulposus. According to the preparation method of the artificial fullerene-carried nucleus pulposus, a semi-molding design concept is adopted, the fullerene nucleus pulposus can be implanted into an affected part with low invasiveness, and surgery risks and postoperative shifting problems are reduced; by adding fullerene into the artificial nucleus pulposus, the content of free radials in the nucleus pulposus can be reduced, and the purposeof decelerating degeneration of the intervertebral disc is achieved. By designing the artificial nucleus pulposus into a structure with an inner layer containing xerogel and an outer layer provided with an electrostatic spinning membrane, on the one hand, a three-dimensional reticular fiber membrane of the outer layer can be utilized to wrap the xerogel of the inner layer without influencing hydration and expansion of the xerogel, shifting of the xerogel is also avoided, and the overflow risk is reduced; on the other hand, the mass of the filling xerogel and the diameter of the fiber membranecan be adjusted according to actual demands to achieve a better filling effect and better recover the structure of the intervertebral disc. Moreover, the artificial nucleus pulposus is a dry solid and is easy to preserve and long in storage life.

Description

technical field [0001] The invention relates to the field of artificial nucleus pulposus preparation, in particular to a preparation method of a fullerene-loaded artificial nucleus pulposus formula. Background technique [0002] According to statistics, in 2015, the population aged 65 and above in China was 144.34 million, accounting for more than 1 / 10 of the national population. In the past ten years, the population aged 65 and above has increased year by year, which means that senile spinal degenerative diseases are accompanied by the peak of population aging. The upcoming will also show a higher growth trend, resulting in limited mobility and neck, shoulder, low back and leg pain. Intervertebral disc degeneration is the premise and pathological basis of spinal degenerative diseases, and the regeneration capacity of intervertebral disc tissue is limited. Once degeneration occurs, it is difficult to prevent or reverse it. [0003] Studies in recent years have found that th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/08A61L27/20A61L27/24A61L27/18D01D5/00D04H1/728
CPCA61L27/08A61L27/18A61L27/20A61L27/24D01D5/0015D04H1/728A61L2430/38C08L5/08C08L1/284C08L75/04
Inventor 乔之光刘永佳孙彬彬戴尅戎朱邦尚赵杰
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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