NADPH-cytochrome P450 reducing ferment and application thereof

A technology of nicotinamide adenine and cytochrome, which is applied in the fields of biotechnology and plant biology, can solve the problems that the catalytic activity needs to be improved and the kinship is far away.
CN104894077AActive Publication Date: 2015-09-09SYNBIOTECH (SUZHOU) CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SYNBIOTECH (SUZHOU) CO LTD
Publication Date
2015-09-09

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Abstract

Provided is a nicotinamide adenine dinucleotide (NADPH)-cytochrome P450 reductase and a use thereof. Also provided is a polynucleotide encoding the NADPH-cytochrome P450 reductase, an expression vector and a host cell which express the NADPH-cytochrome P450 reductase, and a method for producing the protopanoxadiol.
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Description

technical field

[0001] The present invention relates to the field of biotechnology and plant biology; more specifically, the present invention relates to nicotinamide adenine dinucleotide (NADPH)-cytochrome P450 reductase and application thereof. Background technique

[0002] Nicotinamide adenine dinucleotide (NADPH)-cytochrome P450 reductase (EC1.6.2.4Cytochrome P450 Reductase, CPR) is an important part of the cytochrome P450 (CYP) catalytic system. The redox reaction catalyzed by cytochrome P450 requires the supply of electrons (reducing power). NADPH-cytochrome P450 reductase can provide reducing power for the reaction catalyzed by CYP by obtaining electrons from electron donors and transferring them to CYP. In the absence of CPR, CYP has no catalytic activity on the substrate. NADPH-cytochrome P450 reductase belongs to the riboflavin protein family, is a membrane protein and has a conserved domain that binds to the coenzymes FMN, FAD and NADPH.

[0003] Since the domai...

Claims

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