Escherichia coli genetic engineering strain and construction method for synthesizing dammarenediol

A technology of genetically engineered strains and dammarediol, applied in the biological field, can solve the problem of no biosynthesis of dammarediol, etc., and achieve the effects of easy metabolic engineering transformation and simple pathway regulation.
CN105176905BActive Publication Date: 2018-05-08TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2018-05-08

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Abstract

The invention discloses an Escherichia coli genetic engineering strain for synthesizing dammarenediol and a construction method. The Escherichia coli genetic engineering strain for synthesizing dammarenediol contains a squalene synthase gene with 26 amino acid residues truncated at the C-terminal, 2 , 3‑oxidized squalene synthase gene, cytochrome‑NADPH‑reductase 1 gene and dammarenediol synthase gene in Arabidopsis thaliana truncated by 45 amino acid residues at the C-terminus. The invention successfully constructs the Escherichia coli genetic engineering strain for synthesizing dammarenediol. Compared with other host bacteria, Escherichia coli synthesizes dammarenediol pathway with simple regulation and easy metabolic engineering, which lays the foundation for Escherichia coli fermentation production of dammarane-type saponins.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to an Escherichia coli engineering bacterium for synthesizing dammarenediol and a construction method thereof. Background technique

[0002] Ginseng, as a precious Chinese medicinal material, has always been a research and application hotspot in disease treatment and health care. Ginsenosides are a class of triterpenoids, which are active ingredients in ginseng. According to the different triterpenoid saponins, they can be divided into dammarane-type saponins and oleanane-type saponins. Among them, dammarane-type ginsenosides such as Rb1, Rg3, Rh2, CK and saponin protopanaxadiol have the pharmacological effects of protecting cardiovascular, anti-fatigue, anti-aging, anti-cancer, protecting liver and enhancing immunity. The main raw material for the traditional production of ginsenosides is ginseng. So far, wild ginseng resources in our country are on the verge of extinction...

Claims

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