Dammarenediol synthase gene of panax japonicus var and applications thereof

Abstract

The invention discloses a dammarenediol synthase gene of panax japonicus var and applications thereof. The dammarenediol synthase gene of panax japonicus var is prepared by the following steps: carrying out transcriptome sequencing and Denovo splicing on panax japonicus var rhizome by utilizing the second-generation Sole xaHiSeq2000 for the first time; analyzing and finding out a candidate gene for encoding the dammarenediol synthase (DS) in panax japonicus var and performing in vitro cloning, to finally obtain the dammarenediol synthase gene of panax japonicus var, wherein the sequence of the gene is SEQIDNO.1. The gene is genetically transformed into the panax japonicus var through agrobacterium tumefaciens mediated transformation, to obtain transgenic plant with high content of dammarane saponin, thus providing a technical support for the industrial production of the dammarane saponin.

Description

technical field

[0001] The invention belongs to the field of biological technology, and mainly relates to the cloning and application of a dammarenediol synthase (Dammarenediol synthase, DS) gene in Panax notoginseng. Background technique

[0002] Panax japonicus.C.A.Mey.var.bipinnatifidus (Seem.) C.Y.Wu et K.M.Feng) belongs to the Araliaceae Panax L. plant, is one of the traditional precious medicinal materials, has a high medicinal value Use value, such as heat-clearing and detoxifying, smoothing Qi and invigorating the stomach, promoting blood circulation and eliminating fatigue, nourishing and strengthening, etc. Its main active ingredients are saponins, of which oleanane-type pentacyclic triterpene saponins are the main ones, and at the same time contain a small amount of da Marane-type tetracyclic triterpenoid saponins.

[0003] Panax notoginseng is a species recorded in the Chinese Pharmacopoeia. It has broad application prospects and considerable economic value. At...

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