Recombinant Saccharomyces cerevisiae and construction method for producing dammarenediol and protopanaxadiol using xylose

A technology of Saccharomyces cerevisiae and damarenediol, applied in the field of recombinant Saccharomyces cerevisiae and construction, and can solve problems such as low efficiency
CN108949601BActive Publication Date: 2021-09-03TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2021-09-03

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Abstract

The invention discloses a recombinant Saccharomyces cerevisiae strain and a construction method for producing dammarenediol and protopanaxadiol by using xylose. Through homologous recombination, the promoter of Saccharomyces cerevisiae xylulokinase gene XKS1 is replaced with a promoter Sub P FBA1 , and then introduce xylose reductase XYL1, xylitol dehydrogenase XYL2 expression cassettes, and then increase the activity of transketolase TKL1 and transaldolase TAL1 to obtain recombinant bacterium 1, and then introduce farnesyl diphosphate into recombinant bacterium 1 Farnesyltransferase gene ERG9, squalene monooxygenase gene ERG1 and dammarene diol synthase gene DS were obtained to obtain recombinant bacteria 2, and nicotinamide adenine dinucleotide-hydroxymethyl was introduced into recombinant bacteria 2 Glutaryl-CoA reductase gene NADH-HMGr, farnesyl pyrophosphate synthase ERG20, protopanaxadiol synthase-cytochrome P450 reductase fusion protein gene PPDS-ATR1 were obtained to obtain recombinant bacteria 3, the recombinant brewing wine of the present invention Yeast uses xylose to synthesize dammarenediol and protopanaxadiol.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a recombinant Saccharomyces cerevisiae strain for producing dammarenediol and protopanaxadiol by utilizing xylose, a construction method and application thereof. Background technique

[0002] Xylose is a pentose sugar. Natural D-xylose exists in plants in the form of polysaccharides. No free xylose has been found in nature so far. As the main component of plant cell walls, lignocellulose mainly includes three components: cellulose, hemicellulose and lignin. Lignocellulosic biomass produced through photosynthesis on the earth is more than 100 billion tons every year, which is the most abundant renewable energy substance on the earth. By hydrolyzing lignocellulose, rich monosaccharide substances such as glucose and xylose can be obtained. As the monosaccharide in lignocellulose hydrolyzate, which is second only to glucose, the utilization of xylose has attracted extensive attention o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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