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Detection method of ginseng/pseudo-ginseng-containing traditional Chinese medicine preparation

A technology of traditional Chinese medicine preparation and Panax notoginseng, which is applied in the field of medicine preparation, can solve problems such as difficulty in separation, difficulty in formulating standards for traditional Chinese medicine preparations, affecting the specificity of ginseng and Sanqi, achieve low toxicity, improve clinical use safety and stability, strong specific effect

Active Publication Date: 2017-05-31
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Ginseng and Panax notoginseng are both tonic drugs. They are often used alone or in combination in traditional Chinese medicine preparations. When they are used in combination, they are mostly monarch and minister drugs. The chemical components contained in the two belong to saponins, and the structures are similar. Difficulties in formulating standards for traditional Chinese medicine preparations of Panax notoginseng
[0003] In the 2015 "Chinese Pharmacopoeia", when the TLC identification method was used to identify the traditional Chinese medicine preparations containing ginseng and notoginseng, ginsenoside Rb1, ginsenoside Rg1 and notoginseng saponin R1 were used as reference substances for identification, but in actual work, Since the Rf values ​​of notoginseng saponin R1 and ginsenoside Re are very close, it is difficult to separate them, so that there may be false positive results in which ginseng is used instead of notoginseng or notoginseng instead of ginseng, which affects the exclusive identification of ginseng and notoginseng in traditional Chinese medicine preparations. sex

Method used

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  • Detection method of ginseng/pseudo-ginseng-containing traditional Chinese medicine preparation
  • Detection method of ginseng/pseudo-ginseng-containing traditional Chinese medicine preparation
  • Detection method of ginseng/pseudo-ginseng-containing traditional Chinese medicine preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0061] Experimental example 1: TLC identification of ginseng and notoginseng in Fangchi Dan, a traditional Chinese medicine composition

[0062] 1. Preparation of the test solution: take 20 tablets of Fangchi Dan, remove the coating, grind finely, take 5g of the powder, add 40ml of chloroform, heat and reflux for 1 hour, discard the chloroform, and evaporate the dregs to dry the solvent , add 0.5ml of water to stir and moisten, add 10ml of water-saturated n-butanol, ultrasonically treat for 30 minutes, absorb the supernatant and add 3 times the amount of ammonia test solution, shake well, place layers, take the upper layer and evaporate to dryness, and add 1.5ml of methanol to the residue to make Dissolved as the test solution.

[0063] 2. Prepare the negative sample solution: prepare the finished product without ginseng and Panax notoginseng according to the preparation process of Fangchi Dan, grind it finely, take 5g of powder, add 40ml of chloroform, heat and reflux for 1 h...

experiment example 2

[0093] Experimental example 2: Thin-layer chromatography identification of ginseng and Panax notoginseng in Kangerxin capsules commercially available or Kangerxin capsules extract prepared according to the prescription of the Chinese Pharmacopoeia

[0094] 1. Prepare the test solution: take Kangerxin Capsule Extract, grind it finely, take 5g of powder, add 40ml of chloroform, heat and reflux for 1 hour, discard the chloroform solution, evaporate the dregs to dry the solvent, add 0.5ml of water Stir and moisten, add 10ml of water-saturated n-butanol, sonicate for 30 minutes, absorb the supernatant and add 3 times the amount of ammonia test solution, shake well, place in layers, take the upper layer and evaporate to dryness, add 2ml of methanol to the residue to dissolve, and use it as the test solution product solution.

[0095] 2. Prepare the negative sample solution: prepare the finished product without ginseng and Panax notoginseng according to the preparation process of Kan...

experiment example 3

[0104] Experimental Example 3: TLC identification of ginseng in commercially available Yixinshu Tablets or Yixinshu Tablets extract prepared according to the prescription of the Chinese Pharmacopoeia

[0105] 1. Preparation of the test solution: take the extract of Yixinshu tablets, grind it finely, take 2g of powder, add 40ml of chloroform, heat and reflux for 1 hour, discard the chloroform solution, evaporate the dregs to dry the solvent, add 0.5ml of water Stir and moisten, add 10ml of water-saturated n-butanol, sonicate for 30 minutes, absorb the supernatant and add 3 times the amount of ammonia test solution, shake well, place in layers, take the upper layer and evaporate to dryness, add 2ml of methanol to the residue to dissolve, and use it as the test solution product solution.

[0106] 2. Prepare the negative sample solution: prepare the finished product without ginseng according to the preparation process of Yixinshu Tablets, grind it finely, take 2g of the powder, ad...

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Abstract

The invention provides a detection method of a ginseng / pseudo-ginseng-containing traditional Chinese medicine preparation. The method comprises the following steps: (1) taking a ginseng / pseudo-ginseng-containing traditional Chinese medicine composition or traditional Chinese medicine preparation, and preparing a test sample solution; (2) taking a ginseng / pseudo-ginseng-free finished product, and preparing a negative sample solution; (3) taking a ginsenoside Rb1 reference substance, a ginsenoside Re reference substance, a ginsenoside Rg1 reference substance, a ginsenoside Rf reference substance and a pseudo-ginseng saponin R1 reference substance, respectively adding methanol to prepare a mixed solution containing 0.5-2mg of respective reference substance per 1ml as a reference substance mixed solution; (4) detecting; and (5) analyzing the result. The method can simultaneously indentify ginseng and pseudo-ginseng, and simultaneously identify the index components ginsenoside Rb1, ginsenoside Re, pseudo-ginseng saponin R1, ginsenoside Rf and ginsenoside Rg1. The detection method is simple and easy to implement, and has the characteristics of high specificity and favorable repetitiveness.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to a detection method of a traditional Chinese medicine preparation containing ginseng or notoginseng. Background technique [0002] Ginseng and Panax notoginseng are both tonic drugs. They are often used alone or in combination in traditional Chinese medicine preparations. When they are used in combination, they are mostly monarch and minister drugs. The chemical components contained in the two belong to saponins, and the structures are similar. It is difficult to formulate standards for traditional Chinese medicine preparations of Panax notoginseng. [0003] In the 2015 "Chinese Pharmacopoeia", when the TLC identification method was used to identify the traditional Chinese medicine preparations containing ginseng and notoginseng, ginsenoside Rb1, ginsenoside Rg1 and notoginseng saponin R1 were used as reference substances for identification, but in actual work, Sin...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 周厚成胡昌江张彩虹李文兵黄宇林娟
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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