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A method for determining the content of twelve components in a traditional Chinese medicine composition preparation

A measurement method and composition technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of high cost and long inspection cycle

Active Publication Date: 2017-11-17
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the large number of ingredients in the Chinese medicine composition, the current method has a long inspection period and high cost. Therefore, in order to effectively control the product quality and ensure the public's drug safety, this study uses UPLC- MS determination of calycosin-7-O-glucoside (1), isoquercitrin (2), naringerin (3), hesperidin (4), ginsenoside Re (5), Ginsenosides Rg1 (6), Ginsenosides (7), Ginsenosides Rf (8), Ginsenosides Rb1 (9), Astragaloside IV (10), Ginsenosides Rd (11), Ginsenosides H1 (12) content, to provide a reference for its quality control

Method used

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  • A method for determining the content of twelve components in a traditional Chinese medicine composition preparation
  • A method for determining the content of twelve components in a traditional Chinese medicine composition preparation
  • A method for determining the content of twelve components in a traditional Chinese medicine composition preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Example 1: Preparation of pharmaceutical capsules of the present invention

[0056] prescription:

[0057] Astragalus 450g Aconite 112.5g Ginseng 225g Salvia 225g Tinglizi 150g

[0058] Xiangjiapi 180g Alisma 225g Safflower 90g Polygonatum 75g Tangerine peel 75g

[0059] Preparation:

[0060] (1) Astragalus, Tinglizi, Alisma, ginseng, and Perilla frondosa were extracted with 8 times (weight ratio) 70% ethanol and refluxed for 2 times according to the above prescription, the first time was 3 hours, the second time was 2 hours, combined The extract is filtered, the filtrate is decompressed to recover ethanol, and it is concentrated to 60°C to determine a clear ointment with a relative density of 1.25-1.30 for use;

[0061] (2) Cinnamon sticks and tangerine peel are distilled to extract the volatile oil according to the proportion, the aqueous solution after the oil extraction is filtered, and the residue is decocted with 8 times the amount of water for 1 hour, filtered, and the aqu...

Embodiment 2

[0091] Example 2: Preparation of pharmaceutical tablets of the present invention

[0092] prescription:

[0093] Astragalus 150g Aconite 40g Ginseng 225g Salvia 225g Tinglizi 50g Fragrant Jiapi 180g Alisma 75g Polygonatum odoratum 75g Cinnamon sticks 30g Safflower 90g Tangerine peel 25g

[0094] Preparation:

[0095] (1) Astragalus, Tinglizi, Alisma, ginseng, and Perilla frondosa were extracted with 8 times 70% ethanol and refluxed for 2 times according to the above prescription, the first time was 3 hours, the second time was 2 hours, and the extracts were combined and filtered. After passing, the filtrate is decompressed to recover ethanol, and it is concentrated to 60°C to determine a clear paste with a relative density of 1.25-1.30 for use;

[0096] (2) Cinnamon sticks and tangerine peel were distilled to extract the volatile oil according to the prescription, the aqueous solution after the oil extraction was filtered, and the residue was decocted with 8 times the amount of water f...

Embodiment 3

[0112] Example 3: Preparation of pharmaceutical granules of the present invention

[0113] prescription:

[0114] Astragalus 250g Aconite 112.5g Ginseng 200g Salvia 120g Tinglizi 135g

[0115] Xiangjiapi 150g Alisma 200g Polygonatum 60g Cassia twig 75g Safflower 75g Chenpi 60g

[0116] Preparation method: Prepare granules according to conventional preparation methods.

[0117] The method for determining the content of the granules consists of the following steps:

[0118] A. Preparation of test solution: Take the Chinese medicine composition preparation, grind it finely, accurately weigh 0.1g, place it in a conical flask with stopper, add 20 mL of methanol, weigh it, ultrasonically treat it for 10 minutes, let it cool, and weigh , Make up the lost weight with methanol, shake well, and get it through 0.2μm filter membrane;

[0119] B. Preparation of the standard curve: accurately weigh the reference materials Astragaloside IV, Verbasil-7-O-glucoside, Ginsenoside Rb1, Ginsenoside Re, Ginse...

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Abstract

The invention discloses a UPLC-MS quantitative method for the content of twelve components in a traditional Chinese medicine composition preparation, and measures calycocoside-7-O-glucoside (1) and isoquercitrin (2) in the Chinese medicine composition , naringerin rutin (3), hesperidin (4), ginsenoside Re (5), ginsenoside Rg1 (6), barleytoxin (7), ginsenoside Rf (8), ginsenoside Rb1 (9 ), astragaloside IV (10), ginsenoside Rd (11), and bargoside H1 (12), which belong to the field of component testing of traditional Chinese medicine preparations. The measuring method adopted by the invention has short period, good repeatability and high sensitivity.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and specifically relates to a method for determining the content of each component in a traditional Chinese medicine preparation. Background technique [0002] The traditional Chinese medicine composition is a compound traditional Chinese medicine composed of 11 traditional Chinese medicines such as astragalus, ginseng, Xiangjiapi, cassia twig, etc. It has the effects of benefiting temperature and yang, promoting blood circulation and dredging collaterals, diminishing swelling, etc., and is clinically used for lightening of coronary heart disease and hypertension. , Moderate congestive heart failure and other diseases. Modern research shows that the active ingredients in the traditional Chinese medicine composition are diverse, mainly including flavonoids, saponins, alkaloids, etc. The current research on the traditional Chinese medicine composition mainly focuses on clinical appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 刘敏彦赵韶华刘颖欧阳玥李松乔莉
Owner HEBEI YILING MEDICINE INST
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