Method for quickly quality-detecting and identifying American ginsengs, ginsengs and preparations of American ginsengs and ginsengs

Abstract

The invention discloses a method for quickly quality-detecting and identifying American ginsengs, ginsengs and preparations of American ginsengs and ginsengs. In the method, 70-percent methanol is used as a sample ultrasonic extraction solvent, a C18 chromatographic column is adopted, a system of acetonitrile-tetrahydrofuran-formic acid-water is used as a flowing phase, an electrospray ionization(ESI) ion source is adopted, quick separation, identification and measurement of pseudoginsenoside F11 and ginsenoside Rf which are characteristics components of the American ginsengs and ginsengs respectively are performed within 2 minutes for the first time under a condition of a negative ion mode second order mass spectrometry analysis, and whether the preparation of the American ginsengs is mixed with the ginsengs is judged and the content of crude drugs mixed with the ginsengs is worked out. The method has the advantages of strong specificity, synchronous qualitative and quantitative analysis, easy and quick pre-treatment, quick detection of the contents of the American ginsengs and the ginsengs within 2 minutes, high sensitivity of detection and measurement and good repeatability, and the suitability for quick quality screening of the American ginsengs, the ginsengs and products of the American ginsengs and the ginsengs.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicinal materials, and relates to the quality detection of traditional Chinese medicines and health care products, especially the rapid identification and quantitative determination of Panax ginseng and its preparations by using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry Methods. Background technique [0002] American ginseng (Panax quinque folium L.) and ginseng (Panax ginseng C.A.Mey) belong to Araliaceae. American ginseng is mainly produced in North America. It has the functions of nourishing qi and nourishing yin, clearing away heat and promoting body fluid. There are also introduced species in Northeast my country, commonly known as species of American ginseng. Ginseng is a perennial herbaceous plant, mainly produced in Northeast my country. It has the functions of nourishing vital energy, recovering pulse and strength...

AI Technical Summary

Problems solved by technology

The main components of both are ginsenoside compounds, but the efficacy and price are quite different

However, pseudo-ginsenoside F11 and ginsenoside Rf are isomers with very similar polarity and the same molecular weight, and are generally difficult to separate, resulting in difficulty in distinguishing

Existing analytical methods have complicated sample processing, generally take more than 60 minutes to detect, and the separation effect is not good, which is not conducive to quantitative detection and rapid screening

[0004] The current 2005 edition of "Chinese Pharmacopoeia" adopts the thin-layer identification method for the identification of American ginseng medicinal materials, using American ginseng reference medicinal materials, pseudo-ginsenoside F11 reference substance, ginsenoside Rb1 reference substance, ginsenoside Re reference substance, and ginsenoside Rg1 reference substance For comparison, pseudo-ginsenoside F11 is a characteristic component of American ginseng that is different from ginseng, but this component and ginsenoside Rf contained in ginseng are isomers, and it is difficult to distinguish them by thin-layer chromatography, so this method alone is not enough Identify American Ginseng

Under the content determination item in the standard, only ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were measured by high performance liquid chromatography at a detection wavelength of 203nm, and the characteristic components of American ginseng were not yet involved

And the preparation method of the test solution is complicated, it needs to be heated and refluxed with water-saturated n-butanol for 1.5 hours, let it cool, after replenishing the weight, evaporate to dryness, redissolve with 50% methanol, transfer, and then dilute to constant volume

After separation by C18 column, acetonitrile-0.1% phosphoric acid solution is used as mobile phase, and after gradient elution, the absorbance is measured at 203nm wavelength, and the detection time of single liquid phase needs more than 100min. The method is complicated and time-consuming, and the specificity is not strong, still Difficult to identify and detect American ginseng

The rapid separation and determination method of pseudo-ginsenoside F11 and ginsenoside Rf has not been reported yet

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